Data/hora: 14/06/2024 07:53:23

Biblioteca(s): | Epagri-Sede.
Data corrente: | 27/10/2023
Data da última atualização: | 27/10/2023
Autoria: | MANFIO, C. E.; MARO, L. A. C.; PASSOS, J. F. M.; COSTA, M. D.
Título: | DIAGNÓSTICO DE DOENÇAS UTILIZANDO QPCR EM MUDAS CÍTRICAS REGENERADAS APÓS LIMPEZA CLONAL.
Ano de publicação: | 2023
Fonte/Imprenta: | In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 8., 2023, Florianópolis, SC. Resumos... São Paulo, SP: Sociedade Brasileira de Genética, 2023. p. 52
Idioma: | Inglês

Conteúdo:

The citrus seedlings sector in Santa Catarina has experienced considerable expansion in recent years with an
increase in the number of seedlings produced and also in the number of families involved in the activity. The
lack of important diseases that commonly occur in other states of the federation could put the orchards in Santa
Catarina, the seedling producing region and also the experimental collections kept in the open by Epagri at
risk. In order for the activity to be profitable, whether as a fruit producer or seedling producer, it is essential
that the propagation materials used for the formation of seedlings are free of any pathogens. Therefore, the
diagnosis of diseases is extremely important, and clonal cleaning by micrografting is a primordial tool to ensure
the absence of viruses that are highly harmful to the exploitation of this crop. In view of the above, the objective
of this work was to certify the health of citrus seedlings regenerated after clonal cleaning. To obtain the
seedlings, buds from parent plants of the SCS454 Catarina, SCS455 Reinaldo, SCS 456 Sigmar, SCS 457
Souza and SCS458 Osvino cultivars were used. The qPCR technique was used to verify the micrografting
efficiency. Twenty-one seedlings were regenerated in vitro and had their RNA extracted using the Trizol
protocol. The qPCR reactions for analysis of CEVd (Citrus exocortis viroid) and HSV (Hop stunt viroid)
contained 1 &#956;L of cDNA, 10 &#956;L of master Mix (Sybr Green), 0.35 &#956;M of primers, and water to make up the
final volume of 20 &#956;L, and for the qPCR reactions for psorosis, the protocol by Francesco et al., 2014 was
used. Of the twenty-one seedlings, seventeen showed neither the presence of viroids nor Psorosis. The four
seedlings that were positive did not show both diseases together. Although the four seedlings were not
completely cleaned, they showed only one disease. Important results, since they confirm the efficiency of the
clonal cleaning technique through micrografting in obtaining basic material with phytosanitary quality. The
phytosanitary quality of the citrus seedling produced is a basic requirement for the frank development of the
activity and to place the seedling in Santa Catarina on a differentiated level, precisely because it does not occur
important diseases that are already diagnosed in other states of the federation. In this way, it will be possible
to transfer the benefits of biotechnology to the productive sector for the use of healthy propagation material by
nurserymen in the formation of seedlings and, consequently, contributing to the formation of long-lived
orchards. In this sense, the clonal cleaning technique by means of micrografting was efficient for obtaining
material free of viroids and psorisis.

Thesagro: | Key-words: Citrus spp; micrografting; real-time; seedling production.
Categoria do assunto: | G Melhoramento Genético
URL: | http://intranetdoc.epagri.sc.gov.br/producao_tecnico_cientifica/DOC_59084.pdf
Registro original: | Epagri-Sede (Epagri-Sede)
Exemplares: | Epagri-Sede - (108802-1) - UPC - PL - DD -  -  -  - DIS