Data/hora: 01/06/2024 08:57:38

Biblioteca(s): | Epagri-Sede.
Data corrente: | 05/09/2023
Data da última atualização: | 05/09/2023
Autoria: | MANFIO, C. E.; MARO, L. A. C.; PASSOS, J. F. M.; COSTA, M. D.
Título: | DISEASE DIAGNOSIS USING qPCR IN CITRUS SEEDLINGS REGENERATED AFTER CLONAL CLEANING.
Ano de publicação: | 2023
Fonte/Imprenta: | In: CONGRESSO BRASILEIRO INTERDISCIPLINAR EM CIÊNCIA E TECNOLOGIA, 4., 2023, Online. Resumos.. . Diamantina, MG: UFVJM, 2023.
Idioma: | Inglês

Conteúdo:

The citrus seedlings sector in Santa Catarina has experienced considerable expansion in
recent years with an increase in the number of seedlings produced and also in the number of
families involved in the activity. The lack of important diseases that commonly occur in other
states of the federation could put the orchards in Santa Catarina, the seedling producing region and
also the experimental collections kept in the open by Epagri at risk. In order for the activity to be
profitable, whether as a fruit producer or seedling producer, it is essential that the propagation
materials used for the formation of seedlings are free of any pathogens. Therefore, the diagnosis of
diseases is extremely important, and clonal cleaning by micrografting is a primordial tool to ensure
the absence of viruses that are highly harmful to the exploitation of this crop. In view of the above,
the objective of this work was to certify the health of citrus seedlings regenerated after clonal
cleaning. To obtain the seedlings, buds from parent plants of the SCS454 Catarina, SCS455
Reinaldo, SCS 456 Sigmar, SCS 457 Souza and SCS458 Osvino cultivars were used. The qPCR
technique was used to verify the micrografting efficiency. Twenty-one seedlings were regenerated
in vitro and had their RNA extracted using the Trizol protocol. The qPCR reactions for analysis of
CEVd (Citrus exocortis viroid) and HSV (Hop stunt viroid) contained 1 &#956;L of cDNA, 10 &#956;L of
master Mix (Sybr Green), 0.35 &#956;M of primers, and water to make up the final volume of 20 &#956;L,
and for the qPCR reactions for psorosis, the protocol by Francesco et al., 2014 was used. Of the
twenty-one seedlings, seventeen showed neither the presence of viroids nor Psorosis. The four
seedlings that were positive did not show both diseases together. Although the four seedlings were
not completely cleaned, they showed only one disease. Important results, since they confirm the
efficiency of the clonal cleaning technique through micrografting in obtaining basic material with
phytosanitary quality. The phytosanitary quality of the citrus seedling produced is a basic
requirement for the frank development of the activity and to place the seedling in Santa Catarina
on a differentiated level, precisely because it does not occur important diseases that are already
diagnosed in other states of the federation. In this way, it will be possible to transfer the benefits of
biotechnology to the productive sector for the use of healthy propagation material by nurserymen
in the formation of seedlings and, consequently, contributing to the formation of long-lived
orchards. In this sense, the clonal cleaning technique by means of micrografting was efficient for
obtaining material free of viroids and psorisis.

Thesagro: | Citrus spp; micrografting; real-time; seedling production.
Categoria do assunto: | F Plantas e Produtos de Origem Vegetal
URL: | http://intranetdoc.epagri.sc.gov.br/producao_tecnico_cientifica/DOC_58546.pdf
Registro original: | Epagri-Sede (Epagri-Sede)
Exemplares: | Epagri-Sede - (108565-1) - UPC - PL - DD -  -  -  - DIS