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| 9. |  | ROMEIRO, R. da S.; MOURA, A. B.; OLIVEIRA, J. R. de; SILVA, H. S. A.; BARBOSA, L. S.; SOARES, F. M. P.; PERES, F. Evidences of constitutive multiple resistance to antibiotics in some plant pathogenic bacteria. Summa Phytopathologica, Jaboticabal, v. 24, n. 3/4, p. 220-225, jul./dez. 1998.| Biblioteca(s): Epagri-Sede. |
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| 11. | | TOLEDO, V. de A. A. de; NEVES, C. A.; ALVES, E.M.; OLIVEIRA, J. R. de; RUVOLO-TAKASUSUKI, M. C. C.; FAQUINELLO, P. Produção de geleia real em colônias de abelhas africanizadas considerando diferentes suplementos proteicos e a influência de fatores ambientais. Acta Scientiarum: Animal Sciences, Maringá, PR, v. 32, n. 1, p. 101-108, jan./mar. 2010.| Biblioteca(s): Epagri-Sede. |
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| 12. | | LIMA, H. E. de; NECHET, K. de L.; VIEIRA, B. de A. H.; OLIVEIRA, J. R. de; DUARTE, H. da S. S.; QUEIROZ, E. de S.; OLIVEIRA, F. de L. Elaboração e validação de escalas diagramáticas para avaliação da severidade da mancha-bacteriana do feijão - caupi em cultivares com trifólios morfologicamente distintos. Ciência Rural, Santa Maria, RS, v. 43, n. 10, p. 1735-1743, out. 2013.| Biblioteca(s): Epagri-Sede. |
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Registro Completo
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Biblioteca(s): |
Epagri-Sede. |
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Data corrente: |
15/12/2022 |
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Data da última atualização: |
15/12/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GORAYEB, E. S.; ALBUQUERQUE, M. R. M.; FERREIRA, J.; NASCIMENTO, S. C.; SILVA, T. S.; SAVARIS, D. M.; RIBEIRO, L. P.; SILVA, M. C. C. R.; SILVA, F. N. |
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Título: |
Nucleic acid extraction and multiplex analysis for simultaneous detection of the corn stunt complex pathogens in plant and insect tissues. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Tropical Plant Pathology, Viçosa, MG, 2022. |
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Idioma: |
Inglês |
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Conteúdo: |
The corn stunting complex (CSC), composed of two Mollicutes (phytoplasma and spiroplasma) and one marafivirus (maize rayado fino virus, MRFV), is the most important phytosanitary problem of maize crops in Brazil. Currently, MRFV detection is performed separately from mollicutes, which contributes to the omission of virus detection in CSC monitoring activities and basic studies. Here, a complete diagnostic method for simultaneous detection of CSC pathogens is presented, comprising the optimization of an RNA/DNA extraction protocol and a triplex PCR, in which a newly designed MRFV-specific primer pair was included along with the widely used R16F2n/R2 and CSSF2/CSSR6 primers for the mollicutes. As a result, a newly designed MRFV-specific primer targeting the 5′ end of the polyprotein gene was developed and efficiently amplified MRFV from different geographical locations and tissues (plant and insect) in the multiplex reaction. The designed MRFV primer pair was species-specific, sensitive, and did not form secondary structures or dimerize with each other, nor with the other primers used to detect the mollicutes. This method proved to be effective, cheaper, faster, and easier to perform than other methods previously reported. Thus, it is a helpful tool for both basic and applied studies as well as monitoring programs aiming to understand the MRFV presence in the CSC and maize crops. |
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Thesagro: |
enfezamento; Espiroplasma; fitoplasma. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02176naa a2200253 a 4500
001 1132923
005 2022-12-15
008 2022 bl uuuu u00u1 u #d
100 1 $aGORAYEB, E. S.
245 $aNucleic acid extraction and multiplex analysis for simultaneous detection of the corn stunt complex pathogens in plant and insect tissues.$h[electronic resource]
260 $c2022
520 $aThe corn stunting complex (CSC), composed of two Mollicutes (phytoplasma and spiroplasma) and one marafivirus (maize rayado fino virus, MRFV), is the most important phytosanitary problem of maize crops in Brazil. Currently, MRFV detection is performed separately from mollicutes, which contributes to the omission of virus detection in CSC monitoring activities and basic studies. Here, a complete diagnostic method for simultaneous detection of CSC pathogens is presented, comprising the optimization of an RNA/DNA extraction protocol and a triplex PCR, in which a newly designed MRFV-specific primer pair was included along with the widely used R16F2n/R2 and CSSF2/CSSR6 primers for the mollicutes. As a result, a newly designed MRFV-specific primer targeting the 5′ end of the polyprotein gene was developed and efficiently amplified MRFV from different geographical locations and tissues (plant and insect) in the multiplex reaction. The designed MRFV primer pair was species-specific, sensitive, and did not form secondary structures or dimerize with each other, nor with the other primers used to detect the mollicutes. This method proved to be effective, cheaper, faster, and easier to perform than other methods previously reported. Thus, it is a helpful tool for both basic and applied studies as well as monitoring programs aiming to understand the MRFV presence in the CSC and maize crops.
650 $aenfezamento
650 $aEspiroplasma
650 $afitoplasma
700 1 $aALBUQUERQUE, M. R. M.
700 1 $aFERREIRA, J.
700 1 $aNASCIMENTO, S. C.
700 1 $aSILVA, T. S.
700 1 $aSAVARIS, D. M.
700 1 $aRIBEIRO, L. P.
700 1 $aSILVA, M. C. C. R.
700 1 $aSILVA, F. N.
773 $tTropical Plant Pathology, Viçosa, MG, 2022.
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