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![](/consulta/web/img/deny.png) | Acesso ao texto completo restrito à biblioteca da Epagri-Sede. Para informações adicionais entre em contato com biblio@epagri.sc.gov.br. |
Registro Completo |
Biblioteca(s): |
Epagri-Sede. |
Data corrente: |
12/09/2003 |
Data da última atualização: |
12/09/2003 |
Autoria: |
GENOVEZ, M. E.; OLIVEIRA, J. C.; CASTRO, V.; FERRARI, C. I. L; SCARCELLI, E.; CARDOSO, M. V.; PAULIN, L. M.; GREGORY, L.; LANCA NETO, P. |
Título: |
Elevacao na taxa de paricao de rebanho Nelore com leptospirose endemica pelo descarte progressivo e vacinacao. |
Ano de publicação: |
2003 |
Fonte/Imprenta: |
Revista Brasileira de Reproducao Animal, Beho Horizonte, v. 27, n. 3, p. 539-541, jul./set. 2003. |
Idioma: |
Português |
Palavras-Chave: |
Bovino; Doenca animal; Leptospirose; Reproducao; Vacina. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00789naa a2200265 a 4500 001 1026874 005 2003-09-12 008 2003 bl uuuu u00u1 u #d 100 1 $aGENOVEZ, M. E. 245 $aElevacao na taxa de paricao de rebanho Nelore com leptospirose endemica pelo descarte progressivo e vacinacao. 260 $c2003 653 $aBovino 653 $aDoenca animal 653 $aLeptospirose 653 $aReproducao 653 $aVacina 700 1 $aOLIVEIRA, J. C. 700 1 $aCASTRO, V. 700 1 $aFERRARI, C. I. L 700 1 $aSCARCELLI, E. 700 1 $aCARDOSO, M. V. 700 1 $aPAULIN, L. M. 700 1 $aGREGORY, L. 700 1 $aLANCA NETO, P. 773 $tRevista Brasileira de Reproducao Animal, Beho Horizonte$gv. 27, n. 3, p. 539-541, jul./set. 2003.
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Biblioteca(s): |
Epagri-Sede. |
Data corrente: |
28/02/2013 |
Data da última atualização: |
28/02/2013 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
-- - C |
Autoria: |
TOMAZELLI, I. B.; TOMAZELLI JÚNIOR, O.; FREITAS, J. B.; FABBIA, L. M.; FILIPINI, T. A.; SILVA, C. M.; BEDIN, J. M.; DUARTE, D. A. M.; SANTOS, A.; BACCARIN, A.; HIGA, L. R. G.; YANO, D. M. Y.; KILLNER, M.; FREZZA, A. L. C.; ABECIA, E. C. G.; TRONCO, V. M.; J¨²NIOR, W. B. |
Afiliação: |
Epagri |
Título: |
Comparison of the BAX System PCR Method to Brazil's Official Method for the Detection of Salmonella in Food, Water, and Environmental Samples. |
Ano de publicação: |
2008 |
Fonte/Imprenta: |
Journal of Food Protection, v. 71, n. 12, p. 2442-2447, 2008. |
Idioma: |
Inglês |
Conteúdo: |
A two-stage study compared the BAX system PCR method with the reference culture method used by the Brazilian Ministry of Agriculture and Food Supply for the detection of Salmonella in food, water, and environmental samples. In stage 1, fish matrix samples (n = 258) were spiked at several levels with Salmonella and a combination of Salmonella and non-Salmonella competitive organisms. Replicates were analyzed by the BAX system PCR method and the reference method with comparable results (sensitivity ¡Ý 97.5%, specificity ¡Ý 83.3%) from both methods at the limit of detection. In stage 2, a total of 1,988 samples with 70 product types were analyzed with both methods. Five laboratories were involved in this study, and the samples used were from routine analyses. The BAX system PCR method was shown to be comparable to the reference method, with a limit of detection of 1.0 to 2.0 CFU/25 g of sample. Analysis of the results obtained in stage 2 and in the combination of stages 1 and 2 for the BAX system showed the following performance: sensitivity ¡Ý 99.0%, specificity ¡Ý 97.2%, false-negative rate ¡Ü 1.1%, and false-positive rate ¡Ü 2.8%. Therefore, the BAX system appears to be equivalent to the reference method, with ¡Ý97.3% agreement. |
Palavras-Chave: |
Brazil; Detection of Salmonella; M¨¦todo; PCR. |
Categoria do assunto: |
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Marc: |
LEADER 02298naa a2200361 a 4500 001 1088405 005 2013-02-28 008 2008 bl uuuu u00u1 u #d 100 1 $aTOMAZELLI, I. B. 245 $aComparison of the BAX System PCR Method to Brazil's Official Method for the Detection of Salmonella in Food, Water, and Environmental Samples. 260 $c2008 520 $aA two-stage study compared the BAX system PCR method with the reference culture method used by the Brazilian Ministry of Agriculture and Food Supply for the detection of Salmonella in food, water, and environmental samples. In stage 1, fish matrix samples (n = 258) were spiked at several levels with Salmonella and a combination of Salmonella and non-Salmonella competitive organisms. Replicates were analyzed by the BAX system PCR method and the reference method with comparable results (sensitivity ¡Ý 97.5%, specificity ¡Ý 83.3%) from both methods at the limit of detection. In stage 2, a total of 1,988 samples with 70 product types were analyzed with both methods. Five laboratories were involved in this study, and the samples used were from routine analyses. The BAX system PCR method was shown to be comparable to the reference method, with a limit of detection of 1.0 to 2.0 CFU/25 g of sample. Analysis of the results obtained in stage 2 and in the combination of stages 1 and 2 for the BAX system showed the following performance: sensitivity ¡Ý 99.0%, specificity ¡Ý 97.2%, false-negative rate ¡Ü 1.1%, and false-positive rate ¡Ü 2.8%. Therefore, the BAX system appears to be equivalent to the reference method, with ¡Ý97.3% agreement. 653 $aBrazil 653 $aDetection of Salmonella 653 $aM¨¦todo 653 $aPCR 700 1 $aTOMAZELLI JÚNIOR, O. 700 1 $aFREITAS, J. B. 700 1 $aFABBIA, L. M. 700 1 $aFILIPINI, T. A. 700 1 $aSILVA, C. M. 700 1 $aBEDIN, J. M. 700 1 $aDUARTE, D. A. M. 700 1 $aSANTOS, A. 700 1 $aBACCARIN, A. 700 1 $aHIGA, L. R. G. 700 1 $aYANO, D. M. Y. 700 1 $aKILLNER, M. 700 1 $aFREZZA, A. L. C. 700 1 $aABECIA, E. C. G. 700 1 $aTRONCO, V. M. 700 1 $aJ¨²NIOR, W. B. 773 $tJournal of Food Protection$gv. 71, n. 12, p. 2442-2447, 2008.
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