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Registro Completo
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Biblioteca(s): |
Epagri-Sede. |
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Data corrente: |
26/05/2011 |
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Data da última atualização: |
15/06/2011 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
-- - -- |
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Autoria: |
PEREIRA, R. M.; SILVEIRA, E. L.; PEDRINHO, E. A. N.; VAL-MORAES, S. P.; WICKERT, E.; ALVES, L. M. C.; LEMOS, E. G. M. |
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Afiliação: |
Epagri |
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Título: |
Molecular characterization of bacterial populations of diferent soils. |
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Ano de publicação: |
2006 |
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Fonte/Imprenta: |
Brazilian Journal of Microbiology, São Paulo, n. 37, p. 439-447, 2006. |
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Idioma: |
Inglês |
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Conteúdo: |
Until recently, few studies were carried out in Brazil about diversity of bacterial soil communities. Aiming to characterize the bacterial population in the soil through 16S rRNA analysis, two types of soil have been analyzed: one of them characterized by intensive use where tomato, beans and corn were cultivated (CS); the other analyzed soil was under forest (FS), unchanged by man; both located in Guaíra, São Paulo State, Brazil.
Using specific primers, 16S rRNA genes from metagenomic DNA in both soils were amplified by PCR, amplicons were cloned and 139 clones from two libraries were partially sequenced. The use of 16S rRNA analysis allowed identification of several bacterial populations in the soil belonging to the following phyla: Acidobacteria, Actinobacteria, Bacteroidetes, Firmicutes, Proteobacteria Verrucomicrobia in addition to the others that
were not classified, beyond Archaea domain. Differences between FS and CS libraries were observed in size phyla. A larger number of phyla and, consequently, a greater bacterial diversity were found in the under-forest soil. These data were confirmed by the analyses of genetic diversity that have been carried out. The characterization of bacterial communities of soil has made its contribution by providing facts for further studies on the dynamics of bacterial populations in different soil conditions in Brazil.
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Palavras-Chave: |
16S rRNA; Metagenome; Microbial diversity. |
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Categoria do assunto: |
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Marc: |
LEADER 01827naa a2200157 a 4500
001 1076846
005 2011-06-15
008 2006 bl uuuu u00u1 u #d
100 1 $aEpagri
245 $aMolecular characterization of bacterial populations of diferent soils.
260 $c2006
520 $aUntil recently, few studies were carried out in Brazil about diversity of bacterial soil communities. Aiming to characterize the bacterial population in the soil through 16S rRNA analysis, two types of soil have been analyzed: one of them characterized by intensive use where tomato, beans and corn were cultivated (CS); the other analyzed soil was under forest (FS), unchanged by man; both located in Guaíra, São Paulo State, Brazil. Using specific primers, 16S rRNA genes from metagenomic DNA in both soils were amplified by PCR, amplicons were cloned and 139 clones from two libraries were partially sequenced. The use of 16S rRNA analysis allowed identification of several bacterial populations in the soil belonging to the following phyla: Acidobacteria, Actinobacteria, Bacteroidetes, Firmicutes, Proteobacteria Verrucomicrobia in addition to the others that were not classified, beyond Archaea domain. Differences between FS and CS libraries were observed in size phyla. A larger number of phyla and, consequently, a greater bacterial diversity were found in the under-forest soil. These data were confirmed by the analyses of genetic diversity that have been carried out. The characterization of bacterial communities of soil has made its contribution by providing facts for further studies on the dynamics of bacterial populations in different soil conditions in Brazil.
653 $a16S rRNA
653 $aMetagenome
653 $aMicrobial diversity
773 $tBrazilian Journal of Microbiology, São Paulo$gn. 37, p. 439-447, 2006.
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