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Registro Completo |
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Biblioteca(s): |
Epagri-Sede. |
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Data corrente: |
10/08/2011 |
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Data da última atualização: |
10/08/2011 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
GERGER, R. P. C.; FORELL, F.; MEZZALIRA, J. C.; ZAGO, F. C.; VIEIRA, F. K.; MACHADO JÚNIOR, J.; OHLWEILER, L. U.; RAUBER, L. P.; RODRIGUES, J. L. R.; AMBRÓSIO, C. E.; MIGLINO, M. A.; MEZZALIRA, A.; BERTOLINI, M. |
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Afiliação: |
Epagri |
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Título: |
Effect of Fusion-Activation Interval and Embryo Aggregation on In Vitro and In Vivo Development of Cloned Bovine Embryos Produced by Handmade Cloning. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
In: ANNUAL CONFERENCE OF IETS, 2010, Córdoba, Argentina. Proceedings... Sydney, Austrália: Csiro, 2010. p. 185-186. |
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Idioma: |
Português |
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Conteúdo: |
Despite the rather success of cloning by somatic cell nuclear transfer (SCNT), the efficiency in development to term remains low, with a high rate of losses occurring throughout pregnancy due to faulty reprogramming and conceptus abnormalities. As the ideal fusion-activation interval for optimal nuclear reprogramming after cloning is still ill-defined, the aim of this study was to determine the effect of two distinct fusion-activation intervals and embryo aggregation on in vitro and in vivo development of clone bovine embryos. Increasing the fusion-activation interval to 4 h decreased cleavage but not blastocyst rates in 1 x 100% embryos. Blastocyst rates were lower in 1 x 100% embryos activated 2 h post-fusion. In general, cleavage and blastocyst rates for 2 x 100% embryos were higher than for 1 x 100% embryos (91.5% and 46.0% vs. 74.4% and 31.3%, respectively), irrespective of the activation time. Blastocyst rates for 4 hpf-activated embryos, based on cleavage, were higher than for 2 hpf-activated embryos (50.3% vs. 38.3%, respectively), despite the IVC scheme. Despite differences in in vitro development, pregnancy rates and conceptus development during the first two trimesters of pregnancy were similar between groups. A longer fusion-activation interval (4 hpf) or embryo aggregation scheme (2 x 100%) increased blastocyst yield but did not improve in vivo development nor pregnancy maintenance following the transfer to female recipients in cattle. |
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Palavras-Chave: |
Bovine; Clone; Embryos; Handmade; in vitro; in vivo. |
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Categoria do assunto: |
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LEADER 02099naa a2200193 a 4500
001 1079190
005 2011-08-10
008 2010 bl uuuu u00u1 u #d
100 1 $aEpagri
245 $aEffect of Fusion-Activation Interval and Embryo Aggregation on In Vitro and In Vivo Development of Cloned Bovine Embryos Produced by Handmade Cloning.
260 $c2010
520 $aDespite the rather success of cloning by somatic cell nuclear transfer (SCNT), the efficiency in development to term remains low, with a high rate of losses occurring throughout pregnancy due to faulty reprogramming and conceptus abnormalities. As the ideal fusion-activation interval for optimal nuclear reprogramming after cloning is still ill-defined, the aim of this study was to determine the effect of two distinct fusion-activation intervals and embryo aggregation on in vitro and in vivo development of clone bovine embryos. Increasing the fusion-activation interval to 4 h decreased cleavage but not blastocyst rates in 1 x 100% embryos. Blastocyst rates were lower in 1 x 100% embryos activated 2 h post-fusion. In general, cleavage and blastocyst rates for 2 x 100% embryos were higher than for 1 x 100% embryos (91.5% and 46.0% vs. 74.4% and 31.3%, respectively), irrespective of the activation time. Blastocyst rates for 4 hpf-activated embryos, based on cleavage, were higher than for 2 hpf-activated embryos (50.3% vs. 38.3%, respectively), despite the IVC scheme. Despite differences in in vitro development, pregnancy rates and conceptus development during the first two trimesters of pregnancy were similar between groups. A longer fusion-activation interval (4 hpf) or embryo aggregation scheme (2 x 100%) increased blastocyst yield but did not improve in vivo development nor pregnancy maintenance following the transfer to female recipients in cattle.
653 $aBovine
653 $aClone
653 $aEmbryos
653 $aHandmade
653 $ain vitro
653 $ain vivo
773 $tIn: ANNUAL CONFERENCE OF IETS, 2010, Córdoba, Argentina. Proceedings... Sydney, Austrália: Csiro, 2010. p. 185-186.
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Epagri-Sede (Epagri-Sede) |
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Registro Completo
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Biblioteca(s): |
Epagri-Sede. |
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Data corrente: |
17/03/2011 |
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Data da última atualização: |
21/06/2011 |
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Tipo da produção científica: |
Circular Técnica |
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Circulação/Nível: |
-- - -- |
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Autoria: |
ALMEIDA, E. X. de; HANISCH, A. L.; MIRANDA, M.; DALGALLO, D. |
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Afiliação: |
Epagri |
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Título: |
Pastagem perene de verão: Missioneira-gigante (Axonopus catharinensis, Valls). |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
Florianopolis: Epagri, 2009. |
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Idioma: |
Português |
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Conteúdo: |
São abordados aspectos relativos à origem, características, estabelecimento, preparo do solo, calagem e adubação, consorciação, manejo, qualidade e produção de forragem, rendimento animal, lotação, custos de implantação e disponibilidade de mudas de missioneira-gigante (Axonopus catharinensis, Valls).
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Palavras-Chave: |
Missioneira gigante; Pastagem; Variedade. |
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Categoria do assunto: |
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LEADER 00704nam a2200145 a 4500
001 1074972
005 2011-06-21
008 2009 bl uuuu u0uu1 u #d
100 1 $aEpagri
245 $aPastagem perene de verão$bMissioneira-gigante (Axonopus catharinensis, Valls).
260 $aFlorianopolis: Epagri$c2009
520 $aSão abordados aspectos relativos à origem, características, estabelecimento, preparo do solo, calagem e adubação, consorciação, manejo, qualidade e produção de forragem, rendimento animal, lotação, custos de implantação e disponibilidade de mudas de missioneira-gigante (Axonopus catharinensis, Valls).
653 $aMissioneira gigante
653 $aPastagem
653 $aVariedade
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