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Registro Completo |
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Biblioteca(s): |
Epagri-Sede. |
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Data corrente: |
18/02/2016 |
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Data da última atualização: |
18/02/2016 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
LAVINSKI, A. O.; DETMANN, K. C.; SANGLARD, L. M. V.; RODRIGUES, F. A.; ARAUJO, L.; RIBEIRO, D. M.; ARAÚJO, W. L.; MATTA, F. M. |
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Título: |
SILICON ENHANCES ROOT DIAMETER AND AERENCHYMA FORMATION AND INDUCES CHANGES IN METABOLISM AND FERMENTATIVE PATHWAY IN WATERLOGGED RICE PLANTS VIA ETHYLENE-SIGNALING PATHWAYS. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
In: BRAZILIAN CONGRESS OF PLANT PHYSIOLOGY, 15., 2015, Foz do Iguaçu, Paraná, Brazil . Annals... Rio Claro, SP: SBFV, 2015. |
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Idioma: |
Inglês |
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Conteúdo: |
In stagnant culture solutions, we have previously identified altered morphology of roots (i.e.,
thicker and shorter roots) of rice (Oryza sativa L.) plants when supplemented with silicon
(Si). Here, we hypothesized that Si may exert its effects on root morpho-anatomical traits
through a ethylene signaling pathways. To clarify the relationship between Si and root traits
we first conducted a root structural/anatomical characterization of rice plants supplemented
with Si and then the underlying mechanisms were investigated by combining hormonal and
metabolite profiles with expression of a range of genes presumably affected by ethylene. Si
addition did not alter root biomass, but led to decreases in root length together with increased
root diameter. In +Si plants, there was a geater suberization of schlerenchyma and
lignification of the endodermis and exodermis, whereas in ?Si plants differential lignification
was only observed in the exodermis. Si was also associated with remarkable increases in the
cross-sectional areas of vasculature (93%), xylem vessels (50%), cortex (92%) and
particularly aerenchyma (448%). Relative to ?Si plants, their + Si counterparts displayed
lower (80%) concentration of ACC (the immediate ethylene precursor) in parallel to
remarkable higher concentrations of ethylene (600%), salicylic acid (300%) and jasmonic
acid (2500%), whereas the pools of indol-3-acetic acid (auxin) and abscisic acid were
unresponsive to Si. In the +Si plants, we observed a global up-regulation of transcripts from
genes associated with ethylene biosynthesis (in some cases exceeding 200 times, e.g., ACC
oxidase and the homologue for ACC synthase). Overall, Si nutrition caused an up-regulation
of a range of genes associated with synthesis of lignin and suberin and genes associated with
acclimation responses of rice to submergence whose expressions are triggered by ethylene,
with significantly higher abundance for Sub1B (10 fold) and SK1 (100 fold). Overall, Si did
not affect the root levels of sugars, but it reduced the levels of organic acids associated with
the tricarboxylic acid cycle and increased a range of amino acids. Our data suggest an
upregulation of fermentative pathway, in line with an up-regulation of genes encoding for
enzymes related to the anaerobic pathway. However, increases in fermentative pathway seem
not to be associated with hypoxic conditions but they could simply be an additional route to
supply energy that is required for several energy-consuming processes that seem strongly
upregulated in the +Si plants such as biosynthesis of cell wall components. In summary, Si
apparently triggers ethylene production which in turn seems to induce a range of molecular
and metabolic alterations that culminate with the observed changes in root anatomy upon Si
supplementation. MenosIn stagnant culture solutions, we have previously identified altered morphology of roots (i.e.,
thicker and shorter roots) of rice (Oryza sativa L.) plants when supplemented with silicon
(Si). Here, we hypothesized that Si may exert its effects on root morpho-anatomical traits
through a ethylene signaling pathways. To clarify the relationship between Si and root traits
we first conducted a root structural/anatomical characterization of rice plants supplemented
with Si and then the underlying mechanisms were investigated by combining hormonal and
metabolite profiles with expression of a range of genes presumably affected by ethylene. Si
addition did not alter root biomass, but led to decreases in root length together with increased
root diameter. In +Si plants, there was a geater suberization of schlerenchyma and
lignification of the endodermis and exodermis, whereas in ?Si plants differential lignification
was only observed in the exodermis. Si was also associated with remarkable increases in the
cross-sectional areas of vasculature (93%), xylem vessels (50%), cortex (92%) and
particularly aerenchyma (448%). Relative to ?Si plants, their + Si counterparts displayed
lower (80%) concentration of ACC (the immediate ethylene precursor) in parallel to
remarkable higher concentrations of ethylene (600%), salicylic acid (300%) and jasmonic
acid (2500%), whereas the pools ... Mostrar Tudo |
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Palavras-Chave: |
Flooding; Gene expression; Rice; Silicon. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 03883naa a2200253 a 4500
001 1124856
005 2016-02-18
008 2015 bl uuuu u00u1 u #d
100 1 $aLAVINSKI, A. O.
245 $aSILICON ENHANCES ROOT DIAMETER AND AERENCHYMA FORMATION AND INDUCES CHANGES IN METABOLISM AND FERMENTATIVE PATHWAY IN WATERLOGGED RICE PLANTS VIA ETHYLENE-SIGNALING PATHWAYS.$h[electronic resource]
260 $c2015
520 $aIn stagnant culture solutions, we have previously identified altered morphology of roots (i.e., thicker and shorter roots) of rice (Oryza sativa L.) plants when supplemented with silicon (Si). Here, we hypothesized that Si may exert its effects on root morpho-anatomical traits through a ethylene signaling pathways. To clarify the relationship between Si and root traits we first conducted a root structural/anatomical characterization of rice plants supplemented with Si and then the underlying mechanisms were investigated by combining hormonal and metabolite profiles with expression of a range of genes presumably affected by ethylene. Si addition did not alter root biomass, but led to decreases in root length together with increased root diameter. In +Si plants, there was a geater suberization of schlerenchyma and lignification of the endodermis and exodermis, whereas in ?Si plants differential lignification was only observed in the exodermis. Si was also associated with remarkable increases in the cross-sectional areas of vasculature (93%), xylem vessels (50%), cortex (92%) and particularly aerenchyma (448%). Relative to ?Si plants, their + Si counterparts displayed lower (80%) concentration of ACC (the immediate ethylene precursor) in parallel to remarkable higher concentrations of ethylene (600%), salicylic acid (300%) and jasmonic acid (2500%), whereas the pools of indol-3-acetic acid (auxin) and abscisic acid were unresponsive to Si. In the +Si plants, we observed a global up-regulation of transcripts from genes associated with ethylene biosynthesis (in some cases exceeding 200 times, e.g., ACC oxidase and the homologue for ACC synthase). Overall, Si nutrition caused an up-regulation of a range of genes associated with synthesis of lignin and suberin and genes associated with acclimation responses of rice to submergence whose expressions are triggered by ethylene, with significantly higher abundance for Sub1B (10 fold) and SK1 (100 fold). Overall, Si did not affect the root levels of sugars, but it reduced the levels of organic acids associated with the tricarboxylic acid cycle and increased a range of amino acids. Our data suggest an upregulation of fermentative pathway, in line with an up-regulation of genes encoding for enzymes related to the anaerobic pathway. However, increases in fermentative pathway seem not to be associated with hypoxic conditions but they could simply be an additional route to supply energy that is required for several energy-consuming processes that seem strongly upregulated in the +Si plants such as biosynthesis of cell wall components. In summary, Si apparently triggers ethylene production which in turn seems to induce a range of molecular and metabolic alterations that culminate with the observed changes in root anatomy upon Si supplementation.
653 $aFlooding
653 $aGene expression
653 $aRice
653 $aSilicon
700 1 $aDETMANN, K. C.
700 1 $aSANGLARD, L. M. V.
700 1 $aRODRIGUES, F. A.
700 1 $aARAUJO, L.
700 1 $aRIBEIRO, D. M.
700 1 $aARAÚJO, W. L.
700 1 $aMATTA, F. M.
773 $tIn: BRAZILIAN CONGRESS OF PLANT PHYSIOLOGY, 15., 2015, Foz do Iguaçu, Paraná, Brazil . Annals... Rio Claro, SP: SBFV, 2015.
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| 1. |  | LAVINSKI, A. O.; DETMANN, K. C.; SANGLARD, L. M. V.; RODRIGUES, F. A.; ARAUJO, L.; RIBEIRO, D. M.; ARAÚJO, W. L.; MATTA, F. M. SILICON ENHANCES ROOT DIAMETER AND AERENCHYMA FORMATION AND INDUCES CHANGES IN METABOLISM AND FERMENTATIVE PATHWAY IN WATERLOGGED RICE PLANTS VIA ETHYLENE-SIGNALING PATHWAYS. In: BRAZILIAN CONGRESS OF PLANT PHYSIOLOGY, 15., 2015, Foz do Iguaçu, Paraná, Brazil . Annals... Rio Claro, SP: SBFV, 2015.| Tipo: Resumo em Anais de Congresso |
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