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Registro Completo |
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Biblioteca(s): |
Epagri-Sede. |
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Data corrente: |
12/03/2020 |
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Data da última atualização: |
12/03/2020 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
ANDRADE, A.; NOLDIN, J. A.; PEREIRA, A.; KLABUNDE, G. H. F. |
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Título: |
Detecting Acetyl-Coenzyme A Carboxylase Resistance Gene in Rice. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
In: INTERNATIONAL TEMPERATE RICE CONFERENCE, 7., 2020, Pelotas, RS, Brazil. Abstracts... Pelotas, RS: Embrapa, 2020. p. 160 |
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Idioma: |
Inglês |
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Conteúdo: |
The aryloxyphenoxypropionate herbicides (APPs) are graminicides with excellent control of many grass
weeds species, including weedy rice (Oryza sativa L.). These herbicides block the fatty acid biosynthesis
by inhibiting the enzyme acetyl-CoA carboxylase (ACCase), resulting in the death of susceptible plants.
The induced mutation with gamma rays to rice seeds resulted in lines resistant to APPs herbicides
(Andrade et al., 2018). Plant dose?response assays confirmed the resistance to the APPS herbicides
quizalofop-p-ethyl and haloxyfop-p-methyl (Oliveira Neto et al., 2017). The herbicide resistance in rice
is conferred by a single point mutation with an amino acid substitution of the carboxyl transferase
domain of the ACCase gene. A method based upon allele-specific PCR was developed to detect the SNP
G2027T that causes a tryptophan?cysteine substitution in the gene encoding chloroplastic ACCase in
rice. The protocol was tested in 453 rice samples from a segregant population for validation of the
assay. This technique can be exploited to monitor resistant lines in rice breeding programs to detect
homozygous or heterozygous resistant or usceptible plants. The presence of resistant ACCase allele(s)
can be detected fastly and at low cost and can be used in any molecular biology laboratory with
minimal equipments. |
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Palavras-Chave: |
ACCase inhibitors; herbicide resistance; Oryza sativa L. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 01957naa a2200193 a 4500
001 1129437
005 2020-03-12
008 2020 bl uuuu u00u1 u #d
100 1 $aANDRADE, A.
245 $aDetecting Acetyl-Coenzyme A Carboxylase Resistance Gene in Rice.$h[electronic resource]
260 $c2020
520 $aThe aryloxyphenoxypropionate herbicides (APPs) are graminicides with excellent control of many grass weeds species, including weedy rice (Oryza sativa L.). These herbicides block the fatty acid biosynthesis by inhibiting the enzyme acetyl-CoA carboxylase (ACCase), resulting in the death of susceptible plants. The induced mutation with gamma rays to rice seeds resulted in lines resistant to APPs herbicides (Andrade et al., 2018). Plant dose?response assays confirmed the resistance to the APPS herbicides quizalofop-p-ethyl and haloxyfop-p-methyl (Oliveira Neto et al., 2017). The herbicide resistance in rice is conferred by a single point mutation with an amino acid substitution of the carboxyl transferase domain of the ACCase gene. A method based upon allele-specific PCR was developed to detect the SNP G2027T that causes a tryptophan?cysteine substitution in the gene encoding chloroplastic ACCase in rice. The protocol was tested in 453 rice samples from a segregant population for validation of the assay. This technique can be exploited to monitor resistant lines in rice breeding programs to detect homozygous or heterozygous resistant or usceptible plants. The presence of resistant ACCase allele(s) can be detected fastly and at low cost and can be used in any molecular biology laboratory with minimal equipments.
653 $aACCase inhibitors
653 $aherbicide resistance
653 $aOryza sativa L
700 1 $aNOLDIN, J. A.
700 1 $aPEREIRA, A.
700 1 $aKLABUNDE, G. H. F.
773 $tIn: INTERNATIONAL TEMPERATE RICE CONFERENCE, 7., 2020, Pelotas, RS, Brazil. Abstracts... Pelotas, RS: Embrapa, 2020. p. 160
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| 14. |  | SCHERER, R. F.; BELTRAME, A. B.; MARO, L. A. C.; KLABUNDE, G. H. F. Avaliação de desempenho agronômico de dois novos genótipos de bananeira, subgrupo prata, no primeiro ciclo de cultivo. In: SIMPÓSIO DE FRUTICULTURA DA REGIÃO SUL, 2., 2019, Chapecó. Resumos... Chapecó: UFFS, 2019.| Tipo: Resumo em Anais de Congresso |
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| 16. | | MANFIO, C. E.; PEREIRA, A.; KLABUNDE, G. H. F.; ALVES, D. P.; WAMSER, G. H. AVALIAÇÃO DE MARCADORES MOLECULARES ISSR PARA APLICABILIDADE EM DNA FINGERPRINTING DE CULTIVARES DE CEBOLA. In: SEMINÁRIO INTERINSTITUCIONAL DE ENSINO, PESQUISA E EXTENSÃO, 24., 2019, Cruz Alta. Resumos... Cruz Alta: Universidade de Cruz Alta, 2019.| Tipo: Resumo em Anais de Congresso |
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