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Registro Completo |
Biblioteca(s): |
Epagri-Sede. |
Data corrente: |
27/05/2011 |
Data da última atualização: |
30/05/2011 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
ANDRADE, A.; CELEDON, P.; GUIMARAES, X.; LABATE, C. A. |
Afiliação: |
Epagri |
Título: |
Proteomic analysis of the cambial region in juvenile Eucalyptus grandis at three ages. |
Ano de publicação: |
2007 |
Fonte/Imprenta: |
Proteomics, Alemanha, v. 7, n. 13, p. 2258-2274, 2007. |
Idioma: |
Italiano |
Conteúdo: |
Recent advances in genomics and proteomics have provided an excellent opportunity to understand complex biological processes such as wood formation at the gene and protein levels. The aim of this work was to describe the proteins participating in the processes involved in juvenile wood formation by isolating proteins from the cambial region of Eucalyptus grandis, at three ages of growth (6-month-old seedlings, 3- and 6-year-old trees), and also to identify proteins differentially expressed. Using a 2-D-LC-MS/MS strategy we identified a total of 240 proteins, with 54 corresponding spots being present in at least two ages. Overall, nine proteins classified into the functional categories of metabolism, cellular processes, and macromolecular metabolism showed significant changes in expression. Proteins were classified into seven main functional categories, with metabolism representing 35.2% of the total proteins identified. The comparison of the reference maps showed not only differences in the expression pattern of individual proteins at each age, but also among isoforms. The results described in this paper provide a dynamic view of the proteins involved in the formation of juvenile wood in E. grandis. |
Palavras-Chave: |
Proteoma. |
Categoria do assunto: |
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Marc: |
LEADER 01600naa a2200133 a 4500 001 1076902 005 2011-05-30 008 2007 bl uuuu u00u1 u #d 100 1 $aEpagri 245 $aProteomic analysis of the cambial region in juvenile Eucalyptus grandis at three ages. 260 $c2007 520 $aRecent advances in genomics and proteomics have provided an excellent opportunity to understand complex biological processes such as wood formation at the gene and protein levels. The aim of this work was to describe the proteins participating in the processes involved in juvenile wood formation by isolating proteins from the cambial region of Eucalyptus grandis, at three ages of growth (6-month-old seedlings, 3- and 6-year-old trees), and also to identify proteins differentially expressed. Using a 2-D-LC-MS/MS strategy we identified a total of 240 proteins, with 54 corresponding spots being present in at least two ages. Overall, nine proteins classified into the functional categories of metabolism, cellular processes, and macromolecular metabolism showed significant changes in expression. Proteins were classified into seven main functional categories, with metabolism representing 35.2% of the total proteins identified. The comparison of the reference maps showed not only differences in the expression pattern of individual proteins at each age, but also among isoforms. The results described in this paper provide a dynamic view of the proteins involved in the formation of juvenile wood in E. grandis. 653 $aProteoma 773 $tProteomics, Alemanha$gv. 7, n. 13, p. 2258-2274, 2007.
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