| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Epagri-Sede. Para informações adicionais entre em contato com biblio@epagri.sc.gov.br. |
|
Registro Completo |
|
Biblioteca(s): |
Epagri-Sede. |
|
Data corrente: |
21/07/2011 |
|
Data da última atualização: |
16/09/2011 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
BALDASSARI, R. B.; WICKERT, E.; GOES, A. |
|
Afiliação: |
Epagri |
|
Título: |
Pathogenicity, colony morphology and diversity of isolates of Guignardia citricarpa and G. mangiferae isolated from Citrus spp. |
|
Ano de publicação: |
2009 |
|
Fonte/Imprenta: |
European Journal of Plant Pathology, Wageningen, The Netherlands, v. 120, n. , p. 103-110, 2009. |
|
Idioma: |
Inglês |
|
Notas: |
ISSN, 1573-8469 |
|
Conteúdo: |
In the present study, the pathogenicity of 36 isolates of Guignardia species isolated from asymptomatic ?Tahiti? acid lime fruit peels and leaves, ?Pêra-Rio? sweet orange leaves and fruit peel lesions, and a banana leaf were characterized. For pathogenicity testing, discs of citrus leaves colonized by Phyllosticta citricarpa under controlled laboratory conditions were kept in contact with the peels of fruit that were in susceptible states. In addition, pathogenicity was related to morphological characteristics of colonies on oatmeal (OA) and potato dextrose agar (PDA). This allowed the morphological differentiation between G. citricarpa and G. mangiferae. Polymerase chain reactions (PCRs) were also used to identify non-pathogenic isolates based on primers specific to G. citricarpa. A total of 14 pathogenic isolates were detected during pathogenicity tests. Five of these were obtained from leaf and fruit tissues of the ?Tahiti?, which until this time had been considered resistant to the pathogen. Given that the G. citricarpa obtained from this host was pathogenic, it would be more appropriate to use the term insensitive rather than resistant to categorize G. citricarpa. A non-pathogenic isolate was obtained from lesions characteristic of citrus black spot (CBS), indicating that isolation of Guignardia spp. under these conditions does not necessarily imply isolation of pathogenic strains. This also applied to Guignardia spp. isolates from asymptomatic citrus tissues. Using fluorescent amplified fragment length polymorphism (fAFLP) markers, typically pathogenic isolates were shown to be more closely related to one another than to the non-pathogenic forms, indicating that the non-pathogenic isolates display higher levels of genetic diversity. MenosIn the present study, the pathogenicity of 36 isolates of Guignardia species isolated from asymptomatic ?Tahiti? acid lime fruit peels and leaves, ?Pêra-Rio? sweet orange leaves and fruit peel lesions, and a banana leaf were characterized. For pathogenicity testing, discs of citrus leaves colonized by Phyllosticta citricarpa under controlled laboratory conditions were kept in contact with the peels of fruit that were in susceptible states. In addition, pathogenicity was related to morphological characteristics of colonies on oatmeal (OA) and potato dextrose agar (PDA). This allowed the morphological differentiation between G. citricarpa and G. mangiferae. Polymerase chain reactions (PCRs) were also used to identify non-pathogenic isolates based on primers specific to G. citricarpa. A total of 14 pathogenic isolates were detected during pathogenicity tests. Five of these were obtained from leaf and fruit tissues of the ?Tahiti?, which until this time had been considered resistant to the pathogen. Given that the G. citricarpa obtained from this host was pathogenic, it would be more appropriate to use the term insensitive rather than resistant to categorize G. citricarpa. A non-pathogenic isolate was obtained from lesions characteristic of citrus black spot (CBS), indicating that isolation of Guignardia spp. under these conditions does not necessarily imply isolation of pathogenic strains. This also applied to Guignardia spp. isolates from asymptomatic citrus tissues. Using flu... Mostrar Tudo |
|
Palavras-Chave: |
Citrus latifolia; Citrus sinensis; Phyllosticta capitalensis; Phyllosticta citricarpa. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 02388naa a2200181 a 4500
001 1078600
005 2011-09-16
008 2009 bl --- 0-- u #d
100 1 $aEpagri
245 $aPathogenicity, colony morphology and diversity of isolates of Guignardia citricarpa and G. mangiferae isolated from Citrus spp.
260 $c2009
500 $aISSN, 1573-8469
520 $aIn the present study, the pathogenicity of 36 isolates of Guignardia species isolated from asymptomatic ?Tahiti? acid lime fruit peels and leaves, ?Pêra-Rio? sweet orange leaves and fruit peel lesions, and a banana leaf were characterized. For pathogenicity testing, discs of citrus leaves colonized by Phyllosticta citricarpa under controlled laboratory conditions were kept in contact with the peels of fruit that were in susceptible states. In addition, pathogenicity was related to morphological characteristics of colonies on oatmeal (OA) and potato dextrose agar (PDA). This allowed the morphological differentiation between G. citricarpa and G. mangiferae. Polymerase chain reactions (PCRs) were also used to identify non-pathogenic isolates based on primers specific to G. citricarpa. A total of 14 pathogenic isolates were detected during pathogenicity tests. Five of these were obtained from leaf and fruit tissues of the ?Tahiti?, which until this time had been considered resistant to the pathogen. Given that the G. citricarpa obtained from this host was pathogenic, it would be more appropriate to use the term insensitive rather than resistant to categorize G. citricarpa. A non-pathogenic isolate was obtained from lesions characteristic of citrus black spot (CBS), indicating that isolation of Guignardia spp. under these conditions does not necessarily imply isolation of pathogenic strains. This also applied to Guignardia spp. isolates from asymptomatic citrus tissues. Using fluorescent amplified fragment length polymorphism (fAFLP) markers, typically pathogenic isolates were shown to be more closely related to one another than to the non-pathogenic forms, indicating that the non-pathogenic isolates display higher levels of genetic diversity.
653 $aCitrus latifolia
653 $aCitrus sinensis
653 $aPhyllosticta capitalensis
653 $aPhyllosticta citricarpa
773 $tEuropean Journal of Plant Pathology, Wageningen, The Netherlands$gv. 120, n. , p. 103-110, 2009.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Epagri-Sede (Epagri-Sede) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
|
Voltar
|
|
|
| Registros recuperados : 157 | |
| 112. |  | SILVA, A.C.F.da; MULLER, J.J.V.; MIURA, L. Efeito do espacamento, tamanho e corte do tuberculo-semente sobre o rendimento de batata (Solanum tuberosum L.). In: REUNION NACIONAL DE LA ASOCIACION ARGENTINA DE HORTICULTURA, 9. y REUNION LATINOAMERICANA DE HORTICULTURA, 2., 1986, La Plata, Argentina. Resumenes...La Plata, Argentina: Asociacion Argentina de Horticultura, 1986. p. 56.| Biblioteca(s): Epagri-Sede. |
|   |
| 119. | | SILVA, A.C.F.; YOKOYAMA, S.; MULLER, J.J.V.; VIZZOTTO, V.J.; DITTRICH, R.C. Influencia da epoca de transplante na produtividade de cebola. Horticultura Brasileira, Brasilia, v.3, n.2, p. 34-36, nov. 1985.| Biblioteca(s): Epagri-Sede. |
| |
| Registros recuperados : 157 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
