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Registro Completo |
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Biblioteca(s): |
Epagri-Sede. |
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Data corrente: |
11/08/2011 |
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Data da última atualização: |
11/08/2011 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
ZAGO, F. C.; GAUDENCIO NETO, S.; AGUIAR, L. H.; SCHUTZ, L. F.; FORELL, F.; MARINHO, L. R. S.; MACHADO JÚNIOR, J.; MEZZALIRA, A.; BERTOLINI, M. |
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Afiliação: |
Epagri |
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Título: |
Ovum pickup-in vitro production efficiency for conservation of the flamenga cattle breed. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
In: ANNUAL CONFERENCE OF THE INTERNATIONAL EMBRYO TRANSFER SOCIETY, 2011, Orlando, FL, EUA. Proceedings... Sydney, Australia: CSIRO, 2011. p. 213-214. |
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Idioma: |
Inglês |
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Conteúdo: |
The introduction of more specialized and productive dairy breeds into commercial herds in recent decades has caused a gradual loss of interest in dual purpose breeds. The population of the Flamenga, a dual purpose cattle breed, has also suffered a drastic reduction in the world. Due to its importance to biodiversity, the high risk of loss of the small Brazilian herd, electively selected for milk production and located at one geographic region in Southern Brazil, justifies the need and efforts for genetic conservation of the Flamenga breed. For that, the ovum pick up (OPU) procedure becomes a potential tool for such conservation purposes. Thus, the aim of this study was to evaluate the oocyte and embryo yield potential of the Flamenga breed, as compared with Holstein control counterparts, after in vivo oocyte retrieval followed by in vitro embryo production. Four Flamenga and four Holstein multiparous milking females from the same herd were subjected to weekly ultrasound-guided OPU sections for 10 consecutive weeks. The number of visible follicles/ovary/female was recorded prior to the aspiration of ¡Ý3 mm follicles. Retrieved cumulus-oocyte complexes (COC) were morphologically graded according to Leibfried & First (1979; J. Anim. Sci. 48:76-86), and viable COC (Grades I, II and III) from both breeds and from slaughterhouse ovaries (IVP controls) were used for the in vitro production (IVP) of embryos, based on our established procedures (Vieira et al.,, 2002; Cryobiology 45:91-94). Frozen semen from the same Flamenga breed bull was used in all groups. Quantitative (visible and aspirated follicles, collected COC), and qualitative (COC recovery efficiency, cleavage and blastocyst rates) data were compared between breeds and IVP controls (when pertinent) by the Student and ¦Ö2 tests, respectively, for P<0.05. The pools of visible and aspirated follicles were similar between breeds. However, the COC recovery efficiency was higher for Flamenga than for Holstein females. In vitro embryo development was also different between breeds, with cleavage rate being significantly lower for Holstein than for Flamenga and IVP controls. Blastocyst rates also differed between all treatments. Nonetheless, development to blastocyst, when based on cleavage, was similar between Flamenga and Holstein embryos, but with both breeds still being lower than the IVP controls. Oocyte quality was likely the cause of such differences in embryo developmental potential between groups, with Flamenga females yielding more oocytes and IVP embryos than Holstein cows. In summary, the use of OPU-IVP procedures was demonstrated suitable for the preservation of the Flamenga breed in Southern Brazil. MenosThe introduction of more specialized and productive dairy breeds into commercial herds in recent decades has caused a gradual loss of interest in dual purpose breeds. The population of the Flamenga, a dual purpose cattle breed, has also suffered a drastic reduction in the world. Due to its importance to biodiversity, the high risk of loss of the small Brazilian herd, electively selected for milk production and located at one geographic region in Southern Brazil, justifies the need and efforts for genetic conservation of the Flamenga breed. For that, the ovum pick up (OPU) procedure becomes a potential tool for such conservation purposes. Thus, the aim of this study was to evaluate the oocyte and embryo yield potential of the Flamenga breed, as compared with Holstein control counterparts, after in vivo oocyte retrieval followed by in vitro embryo production. Four Flamenga and four Holstein multiparous milking females from the same herd were subjected to weekly ultrasound-guided OPU sections for 10 consecutive weeks. The number of visible follicles/ovary/female was recorded prior to the aspiration of ¡Ý3 mm follicles. Retrieved cumulus-oocyte complexes (COC) were morphologically graded according to Leibfried & First (1979; J. Anim. Sci. 48:76-86), and viable COC (Grades I, II and III) from both breeds and from slaughterhouse ovaries (IVP controls) were used for the in vitro production (IVP) of embryos, based on our established procedures (Vieira et al.,, 2002; Cryobiology 45:9... Mostrar Tudo |
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Palavras-Chave: |
COC; Conservation; Embryo; Flemish; In vitro; Ovum pickup. |
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Categoria do assunto: |
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Marc: |
LEADER 03315naa a2200193 a 4500
001 1079298
005 2011-08-11
008 2011 bl uuuu u00u1 u #d
100 1 $aEpagri
245 $aOvum pickup-in vitro production efficiency for conservation of the flamenga cattle breed.
260 $c2011
520 $aThe introduction of more specialized and productive dairy breeds into commercial herds in recent decades has caused a gradual loss of interest in dual purpose breeds. The population of the Flamenga, a dual purpose cattle breed, has also suffered a drastic reduction in the world. Due to its importance to biodiversity, the high risk of loss of the small Brazilian herd, electively selected for milk production and located at one geographic region in Southern Brazil, justifies the need and efforts for genetic conservation of the Flamenga breed. For that, the ovum pick up (OPU) procedure becomes a potential tool for such conservation purposes. Thus, the aim of this study was to evaluate the oocyte and embryo yield potential of the Flamenga breed, as compared with Holstein control counterparts, after in vivo oocyte retrieval followed by in vitro embryo production. Four Flamenga and four Holstein multiparous milking females from the same herd were subjected to weekly ultrasound-guided OPU sections for 10 consecutive weeks. The number of visible follicles/ovary/female was recorded prior to the aspiration of ¡Ý3 mm follicles. Retrieved cumulus-oocyte complexes (COC) were morphologically graded according to Leibfried & First (1979; J. Anim. Sci. 48:76-86), and viable COC (Grades I, II and III) from both breeds and from slaughterhouse ovaries (IVP controls) were used for the in vitro production (IVP) of embryos, based on our established procedures (Vieira et al.,, 2002; Cryobiology 45:91-94). Frozen semen from the same Flamenga breed bull was used in all groups. Quantitative (visible and aspirated follicles, collected COC), and qualitative (COC recovery efficiency, cleavage and blastocyst rates) data were compared between breeds and IVP controls (when pertinent) by the Student and ¦Ö2 tests, respectively, for P<0.05. The pools of visible and aspirated follicles were similar between breeds. However, the COC recovery efficiency was higher for Flamenga than for Holstein females. In vitro embryo development was also different between breeds, with cleavage rate being significantly lower for Holstein than for Flamenga and IVP controls. Blastocyst rates also differed between all treatments. Nonetheless, development to blastocyst, when based on cleavage, was similar between Flamenga and Holstein embryos, but with both breeds still being lower than the IVP controls. Oocyte quality was likely the cause of such differences in embryo developmental potential between groups, with Flamenga females yielding more oocytes and IVP embryos than Holstein cows. In summary, the use of OPU-IVP procedures was demonstrated suitable for the preservation of the Flamenga breed in Southern Brazil.
653 $aCOC
653 $aConservation
653 $aEmbryo
653 $aFlemish
653 $aIn vitro
653 $aOvum pickup
773 $tIn: ANNUAL CONFERENCE OF THE INTERNATIONAL EMBRYO TRANSFER SOCIETY, 2011, Orlando, FL, EUA. Proceedings... Sydney, Australia: CSIRO, 2011. p. 213-214.
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Registro original: |
Epagri-Sede (Epagri-Sede) |
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| Registros recuperados : 8 | |
| 1. |  | ZAGO, F. C.; GAUDÊNCIO NETO, S.; MARINHO, L. R. S.; SCHUTZ, L. F.; AGUIAR, L. H.; ZARDO, V. F.; MEZZALIRA, A.; BERTOLINI, M. Aspiração folicular transvaginal como ferramenta para obtenção de gametas em vacas da raça flamenga (dados preliminares). In: SEMINÁRIO REGIONAL DOS PÓS-GRADUANDOS EM CIÊNCIAS AGRÁRIAS, 3., 2010, Lages, SC. Anais... Lages, SC: UDESC, 2010.| Tipo: Resumo em Anais de Congresso | Circulação/Nível: -- - -- |
| Biblioteca(s): Epagri-Sede. |
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| 2. | | ZAGO, F. C.; GAUDENCIO NETO, S.; AGUIAR, L. H.; SCHUTZ, L. F.; FORELL, F.; MARINHO, L. R. S.; MACHADO JÚNIOR, J.; MEZZALIRA, A.; BERTOLINI, M. Ovum pickup-in vitro production efficiency for conservation of the flamenga cattle breed. In: ANNUAL CONFERENCE OF THE INTERNATIONAL EMBRYO TRANSFER SOCIETY, 2011, Orlando, FL, EUA. Proceedings... Sydney, Australia: CSIRO, 2011. p. 213-214.| Tipo: Resumo em Anais de Congresso | Circulação/Nível: -- - -- |
| Biblioteca(s): Epagri-Sede. |
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| 3. | | MEZZALIRA, J. C.; OHLWEILER, L. U.; URIO, M.; GAUDENCIO NETO, S.; MARINHO, L. R. S.; ZAGO, F. C.; FORELL, F.; BERTOLINI, M.; MEZZALIRA, A. Effect of nitrocooler negative pressure and recovery internal on cryotolerance of bovine in vitroproduced embryos. In: ANNUAL CONFERECE OF IETS, ., 2010, Cordoba, Argentina. Proceedings... Sydney, Australia: CSIRO, 2010. p. 210.| Tipo: Resumo em Anais de Congresso | Circulação/Nível: -- - -- |
| Biblioteca(s): Epagri-Sede. |
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| 4. | | ZAGO, F. C.; GAUDENCIO NETO, S.; MARINHO, L. R. S.; SCHUTZ, L. F.; AGUIAR, L. H.; ZARDO, V. F.; MEZZALIRA, A.; BERTOLINI, M. Efficiency of reproductive technologies applied to the flamenga breed for genetic conservation (PRELIMINARY DATA). In: REUNIAO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIOES, 24., 2010, Porto de Galinhas, PE. Anais... Porto Alegre, RS: UFRGS, 2010. p. 770.| Tipo: Resumo em Anais de Congresso | Circulação/Nível: -- - -- |
| Biblioteca(s): Epagri-Sede. |
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| 5. | | CASALI, R.; ZAGO, F. C.; FEDERLE, M.; DRUM, J. N.; SPONCHIADO, M.; KLEIN, N.; ZANETTI, M. S.; GAUDÊNCIO NETO, S.; MEZZALIRA, A.; COSTA, U. M.; VIEIRA, A. D. Lyophilized seminal plasma improves the motility of frozen RAM semen and increases the cleavage in heterologous IVF. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 25., 2011, Cumbuco, CE . [Proceedings...]. Porto Alegre, RS: UFRGS, 2011. p. 356.| Tipo: Artigo em Anais de Congresso / Nota Técnica | Circulação/Nível: -- - -- |
| Biblioteca(s): Epagri-Sede. |
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| 6. | | GAUDÊNCIO NETO, S.; MARTINS, L. T.; ORTIGARI JÚNIOR, I.; ZAGO, F. C.; CASALI, R.; SANTOS NETO, P. C.; FELTRIN, C.; MAIA FILHO, S. P.; CASTRO, E. V.; NUNES, J. F.; BERTOLINI, M.; MEZZALIRA, A. Adição de plasma seminal equino liofilizado no reduz a taxa de degradação da motilidade do sêmen ovino congelado. In: REUNIÃO ANUAL DA SOCIEDADE BARSILEIRA DE TECNOLOGIA DE EMBRIÔES, 24., 2010, Porto de Galinhas, PE. Anais... Porto Alegre: UFRGS, 2010. p. 782-782.| Tipo: Resumo em Anais de Congresso | Circulação/Nível: -- - -- |
| Biblioteca(s): Epagri-Sede. |
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| 7. | | SCHTZ, L. F.; ZAGO, F. C.; AGUIAR, L. H.; SANTOS NETO, P. C.; MACHADO JÚNIOR, J.; URIO, M.; MARTINS, L. T.; FERNANDES, D.; BRAUN, W.; GAUDÊNCIO NETO, S.; FELTRIN, C.; BERTOLINI, L. R.; COSTA, U. M.; FORELL, F.; BERTOLINI, M. Changes in blood chemistry profiles and association with birthweights in IVF-derived newborn calves. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 25., 2011, Cumbuco, CE. Acta Scientiea Veterinariae. Porto Alegre, RS: UFRGS, 2011. p. 418.| Tipo: Resumo em Anais de Congresso | Circulação/Nível: -- - -- |
| Biblioteca(s): Epagri-Sede. |
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| 8. | | ORTIGARI JÚNIOR, I.; GERGER, R. P. C.; RIBEIRO, E. S.; MEZZALIRA, J. C.; ZAGO, F. C.; GAUDÊNCIO NETO, S.; OHLWEILER, L. U.; MARTINS, L. T.; BERTOLINI, L. R.; FORELL, F.; COSTA, U. M.; LOPES, R. F. F.; RODRIGUES, J. L.; AMBRÓSIO, C. E.; MIGLINO, M. A.; MEZZALIRA, A.; BERTOLINI, M. Efeito da evolução técnica na eficiência na clonagem por handmade cloning em bovinos. In: REUNIÃO ANUAL DA SOCIEDADE BRASILERIA DE TECNOLOGIA DE EMBRIÕES, 24., 2010, Porto de Galinhas, PE. Anais... Porto Alegre, RS: UFRGS, 2010. p. 803.| Tipo: Resumo em Anais de Congresso | Circulação/Nível: -- - -- |
| Biblioteca(s): Epagri-Sede. |
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| Registros recuperados : 8 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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