Data/hora: 16/06/2024 08:45:25

Biblioteca(s): 	Epagri-Sede.	
Data corrente: 	10/08/2011	
Data da última atualização: 	10/08/2011	
Autoria: 	ZAGO, F. C.; GAUDENCIO NETO, S.; MARINHO, L. R. S.; SCHUTZ, L. F.; AGUIAR, L. H.; ZARDO, V. F.; MEZZALIRA, A.; BERTOLINI, M.	
Afiliação: 	Epagri	
Título: 	Efficiency of reproductive technologies applied to the flamenga breed for genetic conservation (PRELIMINARY DATA).	
Ano de publicação: 	2010	
Fonte/Imprenta: 	In: REUNIAO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIOES, 24., 2010, Porto de Galinhas, PE. Anais... Porto Alegre, RS: UFRGS, 2010. p. 770.	
Idioma: 	Português	
Conteúdo: 	The Flamenga cattle breed was originated in France, being introduced in Brazil in 1945. Flamenga is a double purpose breed known to render meat and milk of excellent quality. Such features, associated with the good adaptation to the altiplane, favored the dissemination of the Flamenga breed in the mountain region of the Santa Catarina State. However, the introduction of more specialized breeds in recent decades caused a gradual loss of interest in the Flamenga breed over the years, resulting in a drastic reduction in the herd size, with only about fifty animals still remaining, all located at the Epagri Experimental Station, in Lages, SC. The high risk of loss imposed on this small genetic pool, along with its importance for biodiversity, justifies the need for conservation of the Flamenga breed. Among other reproductive biotechnologies that could be readily applied for such purpose, the Ovum Pick Up (OPU), used for oocyte retrieval, and superovulation (SOV) for the production of embryos, stand out. However, data regarding the oocyte retrieval efficiency by OPU or the embryo yield by SOV for the Flamenga breed are still lacking. The aim of this study was to evaluate the recovery rate of viable oocytes by OPU and the embryo production efficiency following SOV in the Flamenga breed, comparing with Holstein control counterparts. Eight multiparous females from both breeds were subjected to 17 OPU sessions, at weekly intervals, and to three SOV protocols, in a bimonthly interval. The OPU was performed using an ultrasound device (Falcon 100, Pie Medical, Netherlands) and a 6-MHz linear transducer coupled to a transvaginal aspiration guide system for oocyte retrieval. Following the aspiration of all ¡Ã3 mm follicles, the follicular contents from each animal were searched for cumulus-oocyte complexes (COCs), which were morphologically evaluated. A standard decreasing FSH dose treatment was used for the SOV group, with embryo collection performed by nonsurgical procedures on Day 7 after breeding. Data were compared between breeds using the Wilcoxon test (mean number of COCs recovered per female) or by the Tukey test (embryo yield), for p<0.05 (JMP, SAS Institute Inc., USA). No breed differences were observed in embryo yield after SOV, with 9.9 ¡¾ 5.8 and 5.0 ¡¾ 2.7 viable embryos recovered from Flamenga and Holstein females, respectively. However, a significant breed difference was detected in the mean number of viable COCs collected per female, with 7.8 ¡¾ 0.5 and 6.8 ¡¾ 0.6 COCs recovered from Flamengas and Holsteins. A higher number of viable COCs was obtained from Flamenga females than from Holstein cows, demonstrating the breed¢¥s potential for in vitro embryo production for the genetic conservation of the Flamenga breed.	
Palavras-Chave: 	Embryo; Flemish; in vitro; in vivo; OPU; SOV.	
Categoria do assunto: 	--	
URL: 	http://intranetdoc.epagri.sc.gov.br/producao_tecnico_cientifica/DOC_6299.pdf	
Registro original: 	Epagri-Sede (Epagri-Sede)	
Exemplares: 	Epagri-Sede - (82124-1) - UPC - PL - -- -  -  -  - DIS