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Registro Completo |
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Biblioteca(s): |
Epagri-Sede. |
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Data corrente: |
09/11/2018 |
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Data da última atualização: |
09/11/2018 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
FURTADO, W. E.; MARCHIORI, N. C.; MARTINS, M. L. |
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Título: |
Antiparasitic potential of Biogermex against copepodid stages of Lernaea cyprinacea: MIC test. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
In: CONGRESO COLOMBIANO DE ACUICULTURA, 8., 2018, Bogotá. Resumos... Bogotá: World Aquaculture Society, 2018. |
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Idioma: |
Inglês |
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Conteúdo: |
Lernaea cyprinacea L. (Copepoda), popularly known as ?anchor-worm?, is a crustacean ectoparasite with worldwide distribution and extremely common in freshwater aquaculture. Its impacts on fish farming are well documented, with significant losses reported for disease outbreaks that have resulted in high levels of mortality or disgusting appearance to fish consumer. Due to the high and frequently great potential damage in the use of therapeutic drugs in fish farms, increasing attention is being paid to the use of plant products for disease control/prevention in aquaculture. Biogermex® is a biodegradable organic product made from citrus extracts with low environmental impact. It acts by improving fish resistance against stress, having bactericidal and fungicidal properties. Therefore, it emerges as an alternative to conventional treatments that have already been proven to be more harmful to both fish and public health. Current analysis established the Minimum Inhibitory Concentration (MIC) of Biogermex® on parasite larvae (copepods) of L. cyprinacea. Biological material was collected from broodstocks of silver catfish (Rhamdia quelen) reared in an earth pond at the Experimental field of fish farming (Camboriú, Santa Catarina). Thirteen animals parasitized with L. cyprinacea (mean 192 ± 156 parasites per fish, 40 ? 479) were captured, anesthetized with eugenol (75 mg L-1) and euthanized by brain commotion. Parasites were removed from the host and transferred to a petri plaque with distilled water. The egg-sacs were ruptured to release the eggs which hatched into nauplii 24 h after the process. Further, 100 μL of Biogermex® were added to the first well of the flat-bottom microplate of cell culture (96 wells) and 50 μL of distilled water were added as from the second well. A factor 2 series dilution was performed till the 19th well. Finally, 50μL of distilled water with 5 copepods were added to each well. The test included one control group with distilled water only. Microplates were monitored during periods of 60 minutes and 24 h. MIC was determined as the lowest dilution of the agent in which total inhibition of the larvae of the crustacean occurred (total absence of body movements) in all triplicates. The Biogermex® inhibited copepods of L. cyprinacea in concentrations ranging between 61 and 488 ppm, respectively, according to analysis time. Currently, the most efficacious treatment in Brazil against L. cyprinacea is based on commercial solutions with trichlorphon, an insecticide with proved toxicity for fish and authorized for fish farms. Positive results showed the capacity of the Biogermex® as a prophylactic agent in fish. MenosLernaea cyprinacea L. (Copepoda), popularly known as ?anchor-worm?, is a crustacean ectoparasite with worldwide distribution and extremely common in freshwater aquaculture. Its impacts on fish farming are well documented, with significant losses reported for disease outbreaks that have resulted in high levels of mortality or disgusting appearance to fish consumer. Due to the high and frequently great potential damage in the use of therapeutic drugs in fish farms, increasing attention is being paid to the use of plant products for disease control/prevention in aquaculture. Biogermex® is a biodegradable organic product made from citrus extracts with low environmental impact. It acts by improving fish resistance against stress, having bactericidal and fungicidal properties. Therefore, it emerges as an alternative to conventional treatments that have already been proven to be more harmful to both fish and public health. Current analysis established the Minimum Inhibitory Concentration (MIC) of Biogermex® on parasite larvae (copepods) of L. cyprinacea. Biological material was collected from broodstocks of silver catfish (Rhamdia quelen) reared in an earth pond at the Experimental field of fish farming (Camboriú, Santa Catarina). Thirteen animals parasitized with L. cyprinacea (mean 192 ± 156 parasites per fish, 40 ? 479) were captured, anesthetized with eugenol (75 mg L-1) and euthanized by brain commotion. Parasites were removed from the host and transferred to a petri plaque wi... Mostrar Tudo |
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Palavras-Chave: |
fish disease; Lerneosis; phytotherapic; treatment. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 03320naa a2200193 a 4500
001 1127930
005 2018-11-09
008 2018 bl uuuu u00u1 u #d
100 1 $aFURTADO, W. E.
245 $aAntiparasitic potential of Biogermex against copepodid stages of Lernaea cyprinacea$bMIC test.$h[electronic resource]
260 $c2018
520 $aLernaea cyprinacea L. (Copepoda), popularly known as ?anchor-worm?, is a crustacean ectoparasite with worldwide distribution and extremely common in freshwater aquaculture. Its impacts on fish farming are well documented, with significant losses reported for disease outbreaks that have resulted in high levels of mortality or disgusting appearance to fish consumer. Due to the high and frequently great potential damage in the use of therapeutic drugs in fish farms, increasing attention is being paid to the use of plant products for disease control/prevention in aquaculture. Biogermex® is a biodegradable organic product made from citrus extracts with low environmental impact. It acts by improving fish resistance against stress, having bactericidal and fungicidal properties. Therefore, it emerges as an alternative to conventional treatments that have already been proven to be more harmful to both fish and public health. Current analysis established the Minimum Inhibitory Concentration (MIC) of Biogermex® on parasite larvae (copepods) of L. cyprinacea. Biological material was collected from broodstocks of silver catfish (Rhamdia quelen) reared in an earth pond at the Experimental field of fish farming (Camboriú, Santa Catarina). Thirteen animals parasitized with L. cyprinacea (mean 192 ± 156 parasites per fish, 40 ? 479) were captured, anesthetized with eugenol (75 mg L-1) and euthanized by brain commotion. Parasites were removed from the host and transferred to a petri plaque with distilled water. The egg-sacs were ruptured to release the eggs which hatched into nauplii 24 h after the process. Further, 100 μL of Biogermex® were added to the first well of the flat-bottom microplate of cell culture (96 wells) and 50 μL of distilled water were added as from the second well. A factor 2 series dilution was performed till the 19th well. Finally, 50μL of distilled water with 5 copepods were added to each well. The test included one control group with distilled water only. Microplates were monitored during periods of 60 minutes and 24 h. MIC was determined as the lowest dilution of the agent in which total inhibition of the larvae of the crustacean occurred (total absence of body movements) in all triplicates. The Biogermex® inhibited copepods of L. cyprinacea in concentrations ranging between 61 and 488 ppm, respectively, according to analysis time. Currently, the most efficacious treatment in Brazil against L. cyprinacea is based on commercial solutions with trichlorphon, an insecticide with proved toxicity for fish and authorized for fish farms. Positive results showed the capacity of the Biogermex® as a prophylactic agent in fish.
653 $afish disease
653 $aLerneosis
653 $aphytotherapic
653 $atreatment
700 1 $aMARCHIORI, N. C.
700 1 $aMARTINS, M. L.
773 $tIn: CONGRESO COLOMBIANO DE ACUICULTURA, 8., 2018, Bogotá. Resumos... Bogotá: World Aquaculture Society, 2018.
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Registro original: |
Epagri-Sede (Epagri-Sede) |
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| Registros recuperados : 11 | |
| 2. |  | FURTADO, W. E.; CARDOSO, L.; MARCHIORI, N. C.; MARTINS, M. L. Alterações histológicas em tegumento de jundiás Rhamdia quelen (Quoy & Gaimard, 1824) intensamente parasitados por Lernaea cyprinacea Linnaeus, 1758 (Copepoda: Lernaeidae). In: ENCONTRO BRASILEIRO DE PATOLOGISTAS DE ORGANISMOS AQUÁTICOS, 15., 2019, São Paulo. Resumos... Maringá: Abrapoa, 2019.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Epagri-Sede. |
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| 3. | | FURTADO, W. E.; FIGUEIREDO, A. B.; MARCHIORI, N. C.; MARTINS, M. L. Parâmetros hematológicos de jundiás Rhamdia quelen (Quoy & Gaimard, 1824) intensamente parasitados por Lernaea cyprinacea Linnaeus, 1758. In: ENCONTRO BRASILEIRO DE PATOLOGISTAS DE ORGANISMOS AQUÁTICOS, 15., 2018, Rio de Janeiro. Resumos. Rio de Janeiro: Abrapoa, 2018.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Epagri-Sede. |
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| 4. | | TANCREDO, K. R.; MARCHIORI, N. C.; FURTADO, W. E.; MARTINS, M. L. Prevalence of monogenean species in Oreochromis niloticus from southern Brazil. In: INTERNATIONAL SYMPOSIUM ON MONOGENEA, 8., 2017, Brno, Czech Republic. Abstracts... Brno, Czech Republic: ISM, 2017. p. 133| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Epagri-Sede. |
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| 5. | | FURTADO, W. E.; TANCREDO, K. R.; CARDOSO, L.; MARCHIORI, N. C.; MARTINS, M. L. Caracterização morfológica de Lernaea cyprinacea Linnaeus, 1758 coletado de jundiá Rhamdia quelen (Quoy & Gaimard, 1824) naturalmente infectado. In: SEMANA ACADÊMICA DO CURSO DE ENGENHARIA DE AQUICULTURA, 16., 2018, Florianópolis. Resumos... Florianópolis: UFSC, 2018.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Epagri-Sede. |
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| 6. | | FURTADO, W. E.; CARDOSO, L.; FIGUEREDO, A. B.; MARCHIORI, N. C.; MARTINS, M. L. Histological and hematological alterations of silver catfish Rhamdia quelen highly parasitized by Lernaea cyprinacea. Diseases of Aquatic Organisms, Oldendorf, v. 135, n. 2, p. 157-168, 2019.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Epagri-Sede. |
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| 7. | | MARCHIORI, N. C.; FURTADO, W. E.; BERTOLDI, F. C.; SILVA, B. C.; MASSAGO, H.; MARTINS, M. L. Potencial antiparasitário das nanoemulsões de óleos essenciais de acícula e resina de Pinus sp. nos estágios larvais de Lernaea cyprinacea (Copepoda, Lernaeidae). In: SEMANA ACADÊMICA DO CURSO DE ENGENHARIA DE AQUICULTURA DA UFSC, 16., 2018, Florianópolis. Resumos... Florianópolis: UFSC, 2018.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Epagri-Sede. |
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| 8. | | FURTADO, W. E.; MARCHIORI, N. C.; BERTOLDI, F. C.; SILVA, B. C.; MASSAGO, H.; MARTINS, M. L. Potencial antiparasitário dos óleos nanoemulsionados da resina e acícula de Pinus sp. contra os estágios larvais de Lernaea cyprinacea. In: ENCONTRO BRASILEIRO DE PATOLOGISTAS DE ORGANISMOS AQUÁTICOS, 15., 2019, São Paulo. Resumos... Maringá: Abrapoa, 2019.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Epagri-Sede. |
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| 9. | | FURTADO, W. E.; PESSATTI, T. B.; TANCREDO, K. R.; CARDOSO, L.; MARCHIORI, N. C.; MARTINS, M. L. Caracterização morfológica e identificação molecular de Lernaea cyprinacea Linnaeus, 1758 em jundiás Rhamdia quelen (Siluriformes: Heptapteridae) naturalmente parasitados. In: ENCONTRO BRASILEIRO DE PATOLOGISTAS DE ORGANISMOS AQUÁTICOS, 15., 2018, Rio de Janeiro. Resumos... Rio de Janeiro: Abrapoa, 2018.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Epagri-Sede. |
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| 10. | | FURTADO, W. E.; LEHMANN, N. B.; MEDEIROS, P. B.; CARDOSO, L.; MARCHIORI, N. C.; MASSAGO, H.; SILVA, B. C.; BERTOLDI, F. C.; MARTINS, M. L. Avaliação da concentração letal mediana (CL50, 96h) da nanoemulsão de óleo essencial das acículas de Pinus sp. em alevinos de jundiá Rhamdia quelen (Quoy & Gaimard, 1824). In: ENCONTRO BRASILEIRO DE PATOLOGIAS DE ORGANISMOS AQUÁTICOS, 15., 2018, Rio de Janeiro. Resumos... Rio de Janeiro: ABRAPOA, 2018.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Epagri-Sede. |
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| 11. | | FURTADO, W. E.; CARDOSO, L.; MEDEIROS, P. B.; LEHMANN, N. B.; BERTAGLIA, E. A.; MARCHIORI, N. C.; BERTOLDI, F. C.; MARTINS, M. L. Antiparasitic potential of alternative treatments against larval stages of Lernaea cyprinacea. Journal of Parasitic Diseases, Suíça, p. 1-10, 2021.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - B |
| Biblioteca(s): Epagri-Sede. |
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| Registros recuperados : 11 | |
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