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Registro Completo |
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Biblioteca(s): |
Epagri-Sede. |
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Data corrente: |
18/07/2011 |
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Data da última atualização: |
18/07/2011 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
DANTAS, A. C. M.; BONETI, J. I.; SILVA, S. W.; FERRARI, M.; NASCIMENTO, M.C.A, GUERRA, M.P. |
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Afiliação: |
ePAGRI |
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Título: |
Funcional genomics and proteomicas for Colletotrichum gloeosporioides to resistance in apple. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 3., 2011, Ilhéus, BA. Resumos... Ilhéus, BA: Sociedade brasileira de Genética, 2011. p. 2-2. |
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Idioma: |
Inglês |
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Conteúdo: |
The use of molecular markers associated with biotechnology, mining and gene transfer enables significant advances in thedevelopment of new varieties, justifying the studies performed in this work featuring similar resistance genes and studyinggene expression and proteins in apple cultivars Gala (susceptible) and Fuji (resistance) in order to obtain clones resistance toGala leaf spot (GLS), causal agent Colletotrichum gloeosporioides. It was tested 17 degenerate primers of the sequences fromthe P-loop motifs, and GLPL RNBS D-NBS-LRR domain were amplified from four primers combinations: motifs andN-terminal P-loop within the NBS domain, kinase motifs NBS2, Kinase-2 and C-terminal GLPL, and motifs BSL-2 andC-terminal GLPL. These last two motifs cover the NBS and LRR domains. The amplifications were cloned, sequenced andcharacterized by presenting 25 sequences with potential RGAs differentially expressed in resistant and susceptible the GLS. Itwas obtained 16 contigs with homology to resistance genes and a phylogenetic tree was constructed with genetic similarity inthe species Malus baccata, M. floribunda, Pyrus communis, Prunus persica and Arabidopsis thaliana. It was designed 12 pairs ofspecific RGA primers to analyze expression gene performed mRNA extracted from the Gala and Fuji cultivars inoculated atdifferent periods with isolated 197SJ (0, 4, 8, 12 and 24 after inoculation). RGAs were amplified regions expressed Kinase,GLPL-C-terminal P-loop-GKTT, GLPL - non TIR in Arabidopsis. These data revealed that the resistance genes (R) are highlyconserved among plant species, which are defined according to the protein coding domain. Comparative protein profilesusing 2-D gel in the leaf tissues of apple trees inoculated with isolated 197SJ, increased expression of proteins (spots) 8 hoursafter inoculation on cultivar Fuji (resistance), with molecular weights between 80 to 160 kDa which was not observed withcultivar Gala (susceptible). Proteomics regions associated domain PR was efficient in identifying groups of specific proteinsinvolved resistance to Colletotrichum gloeosporioides in apple during the infection process and host defense. MenosThe use of molecular markers associated with biotechnology, mining and gene transfer enables significant advances in thedevelopment of new varieties, justifying the studies performed in this work featuring similar resistance genes and studyinggene expression and proteins in apple cultivars Gala (susceptible) and Fuji (resistance) in order to obtain clones resistance toGala leaf spot (GLS), causal agent Colletotrichum gloeosporioides. It was tested 17 degenerate primers of the sequences fromthe P-loop motifs, and GLPL RNBS D-NBS-LRR domain were amplified from four primers combinations: motifs andN-terminal P-loop within the NBS domain, kinase motifs NBS2, Kinase-2 and C-terminal GLPL, and motifs BSL-2 andC-terminal GLPL. These last two motifs cover the NBS and LRR domains. The amplifications were cloned, sequenced andcharacterized by presenting 25 sequences with potential RGAs differentially expressed in resistant and susceptible the GLS. Itwas obtained 16 contigs with homology to resistance genes and a phylogenetic tree was constructed with genetic similarity inthe species Malus baccata, M. floribunda, Pyrus communis, Prunus persica and Arabidopsis thaliana. It was designed 12 pairs ofspecific RGA primers to analyze expression gene performed mRNA extracted from the Gala and Fuji cultivars inoculated atdifferent periods with isolated 197SJ (0, 4, 8, 12 and 24 after inoculation). RGAs were amplified regions expressed Kinase,GLPL-C-terminal P-loop-GKTT, GLPL - non TIR in Arabid... Mostrar Tudo |
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Palavras-Chave: |
Gala Leaf Spot; resistance to fungus; RGAs; TR-PCR. |
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Categoria do assunto: |
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Marc: |
LEADER 02744naa a2200169 a 4500
001 1078409
005 2011-07-18
008 2011 bl uuuu u00u1 u #d
100 1 $aePAGRI
245 $aFuncional genomics and proteomicas for Colletotrichum gloeosporioides to resistance in apple.
260 $c2011
520 $aThe use of molecular markers associated with biotechnology, mining and gene transfer enables significant advances in thedevelopment of new varieties, justifying the studies performed in this work featuring similar resistance genes and studyinggene expression and proteins in apple cultivars Gala (susceptible) and Fuji (resistance) in order to obtain clones resistance toGala leaf spot (GLS), causal agent Colletotrichum gloeosporioides. It was tested 17 degenerate primers of the sequences fromthe P-loop motifs, and GLPL RNBS D-NBS-LRR domain were amplified from four primers combinations: motifs andN-terminal P-loop within the NBS domain, kinase motifs NBS2, Kinase-2 and C-terminal GLPL, and motifs BSL-2 andC-terminal GLPL. These last two motifs cover the NBS and LRR domains. The amplifications were cloned, sequenced andcharacterized by presenting 25 sequences with potential RGAs differentially expressed in resistant and susceptible the GLS. Itwas obtained 16 contigs with homology to resistance genes and a phylogenetic tree was constructed with genetic similarity inthe species Malus baccata, M. floribunda, Pyrus communis, Prunus persica and Arabidopsis thaliana. It was designed 12 pairs ofspecific RGA primers to analyze expression gene performed mRNA extracted from the Gala and Fuji cultivars inoculated atdifferent periods with isolated 197SJ (0, 4, 8, 12 and 24 after inoculation). RGAs were amplified regions expressed Kinase,GLPL-C-terminal P-loop-GKTT, GLPL - non TIR in Arabidopsis. These data revealed that the resistance genes (R) are highlyconserved among plant species, which are defined according to the protein coding domain. Comparative protein profilesusing 2-D gel in the leaf tissues of apple trees inoculated with isolated 197SJ, increased expression of proteins (spots) 8 hoursafter inoculation on cultivar Fuji (resistance), with molecular weights between 80 to 160 kDa which was not observed withcultivar Gala (susceptible). Proteomics regions associated domain PR was efficient in identifying groups of specific proteinsinvolved resistance to Colletotrichum gloeosporioides in apple during the infection process and host defense.
653 $aGala Leaf Spot
653 $aresistance to fungus
653 $aRGAs
653 $aTR-PCR
773 $tIn: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 3., 2011, Ilhéus, BA. Resumos... Ilhéus, BA: Sociedade brasileira de Genética, 2011. p. 2-2.
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Epagri-Sede (Epagri-Sede) |
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