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Registro Completo |
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Biblioteca(s): |
Epagri-Sede. |
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Data corrente: |
25/05/2011 |
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Data da última atualização: |
25/05/2011 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
LABATE, M.; BERTOLO, A. L. F.; NASCIMENTO, D. D.; GUTMANIS, G.; ANDRADE, A.; RODRIGUES, M. J. C.; CAMARGO, E. L.; BOARETTO, L. F.; MOON, D. H.; BRAGATTO, J.; LABATE, C. A. |
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Afiliação: |
Epagri |
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Título: |
Cloning and endogenous expression of a Eucalyptus grandis UDP-glucose dehydrogenase cDNA. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Genetics and Molecular Biology, v. 33, n. 4, p. 686-695, 2010. |
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Idioma: |
Inglês |
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Conteúdo: |
UDP-glucose dehydrogenase (UGDH) catalyzes the oxidation of UDP-glucose (UDP-Glc) to UDP-glucuronate (UDP-GlcA), a key sugar nucleotide involved in the biosynthesis of plant cell wall polysaccharides. A full-length cDNA fragment coding for UGDH was cloned from the cambial region of 6-month-old E. grandis saplings by RT-PCR. The 1443-bp-ORF encodes a protein of 480 amino acids with a predicted molecular weight of 53 kDa. The
recombinant protein expressed in Escherichia coli catalyzed the conversion of UDP-Glc to UDP-GlcA, confirming that the cloned cDNA encodes UGDH. The deduced amino acid sequence of the cDNA showed a high degree of identity with UGDH from several plant species. The Southern blot assay indicated that more than one copy ofUGDH
is present in Eucalyptus. These results were also confirmed by the proteomic analysis of the cambial region of 3- and 22-year-old E. grandis trees by 2-DE and LC-MS/MS, showing that at least two isoforms are present. The cloned gene is mainly expressed in roots, stem and bark of 6-month-old saplings, with a lower expression in leaves. High expression levels were also observed in the cambial region of 3- and 22-year-old trees. The results described in this paper provide a further view of the hemicellulose biosynthesis during wood formation in E. grandis.
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Palavras-Chave: |
Proteoma. |
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Categoria do assunto: |
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Marc: |
LEADER 01705naa a2200133 a 4500
001 1076837
005 2011-05-25
008 2010 bl uuuu u00u1 u #d
100 1 $aEpagri
245 $aCloning and endogenous expression of a Eucalyptus grandis UDP-glucose dehydrogenase cDNA.
260 $c2010
520 $aUDP-glucose dehydrogenase (UGDH) catalyzes the oxidation of UDP-glucose (UDP-Glc) to UDP-glucuronate (UDP-GlcA), a key sugar nucleotide involved in the biosynthesis of plant cell wall polysaccharides. A full-length cDNA fragment coding for UGDH was cloned from the cambial region of 6-month-old E. grandis saplings by RT-PCR. The 1443-bp-ORF encodes a protein of 480 amino acids with a predicted molecular weight of 53 kDa. The recombinant protein expressed in Escherichia coli catalyzed the conversion of UDP-Glc to UDP-GlcA, confirming that the cloned cDNA encodes UGDH. The deduced amino acid sequence of the cDNA showed a high degree of identity with UGDH from several plant species. The Southern blot assay indicated that more than one copy ofUGDH is present in Eucalyptus. These results were also confirmed by the proteomic analysis of the cambial region of 3- and 22-year-old E. grandis trees by 2-DE and LC-MS/MS, showing that at least two isoforms are present. The cloned gene is mainly expressed in roots, stem and bark of 6-month-old saplings, with a lower expression in leaves. High expression levels were also observed in the cambial region of 3- and 22-year-old trees. The results described in this paper provide a further view of the hemicellulose biosynthesis during wood formation in E. grandis.
653 $aProteoma
773 $tGenetics and Molecular Biology$gv. 33, n. 4, p. 686-695, 2010.
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| Registros recuperados : 2 | |
| 1. |  | NASCIMENTO, D. D.; ANDRADE, A.; LABATE, C. A.; VENEZIANO, L. M. T.; GUTMANIS, G.; BERTOLO, A. L.; BRAGATTO, J Modulating MIOX2 expression in Nicotiana tabacum and impacts on genes involved in cell wall biosynthesis. Bioenergia em Revista, Piracicaba, SP, v. 2, n. 1, p. 60-82, 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: -- - -- |
| Biblioteca(s): Epagri-Sede. |
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| 2. | | LABATE, M.; BERTOLO, A. L. F.; NASCIMENTO, D. D.; GUTMANIS, G.; ANDRADE, A.; RODRIGUES, M. J. C.; CAMARGO, E. L.; BOARETTO, L. F.; MOON, D. H.; BRAGATTO, J.; LABATE, C. A. Cloning and endogenous expression of a Eucalyptus grandis UDP-glucose dehydrogenase cDNA. Genetics and Molecular Biology, v. 33, n. 4, p. 686-695, 2010.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: -- - -- |
| Biblioteca(s): Epagri-Sede. |
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| Registros recuperados : 2 | |
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