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| 1. |  | QUEIROZ, R. P. V.; SANTOS, W. L. M. dos; BARBOSA, H. V.; SOUZA, R. M. de; SANTOS FILHO, A. M. P. dos. A importancia do diagnostico da cisticercose bovina. Revista Higiene Alimentar, Sao Paulo, v. 11, n. 77, p. 12-15, out. 2000.| Biblioteca(s): Epagri-Sede. |
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Registro Completo
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Biblioteca(s): |
Epagri-Sede. |
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Data corrente: |
07/06/2011 |
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Data da última atualização: |
07/06/2011 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
-- - -- |
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Autoria: |
NUNES, M.; PEREIRA, A.; FERREIRA, J. F.; YASUMARU, F. |
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Afiliação: |
Epagri |
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Título: |
Evaluation of the Microalgae Paste Viability Produced in a Mollusk hatchery in Southern Brazil. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
Journal of the World Aquaculture Society, USA, v. 40, n. 1, p. 87-94, 2009. |
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Idioma: |
Inglês |
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Notas: |
ISSN, 1749-7345 |
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Conteúdo: |
The present study was conducted to define a methodology to produce and store small-scale microalgae paste to be used in a mollusk hatchery. Microalgae were cultured in 500 L fiberglass tanks, under temperature of 20 ¡À 2 C, Guillard f/2 culture medium, and continuous light intensity of 203¨C226 ¦Ìmol photons/m2/sec. Cultures were centrifuged at 2000 g at the exponential growth phase. Microalgae cell quality after centrifugation and during storage was determined by analyses with Evan¡¯s blue stain and by counting the number of total marine bacteria. Treatments with and without additive were applied to the microalgae paste produced, which was distributed into 100 mL plastic containers, capped, and stored under refrigeration at 4 ¡À 1 C. Results indicated that in the Chaetoceros muelleri paste, centrifugation did not damage the cells and the number of total marine bacteria reduced significantly from 2.9 ¡Á 106 to 8.3 ¡Á 105 colony-forming units per milliliter. Chaetoceros muelleri and Chaetoceros calcitrans pastes stored with addition of 0.1% ascorbic acid had a shelf life shorter than 2 wk. For the treatment without additive, results with Evan¡¯s blue stain showed that cells (99%) remained viable until the sixth week of storage for C. muelleri and seventh week of storage for Skeletonema sp. and C. calcitrans. The number of bacteria did not increase during storage for C. calcitrans and Skeletonema (P > 0.05). For C. muelleri, an increase in bacteria (P < 0.05) was observed after the sixth week of storage. This study demonstrated the feasibility to produce and store microalgae paste for a period of 2¨C8 wk, which allows it to be used as food source and also optimizes the use of microalgae cultured in laboratory. MenosThe present study was conducted to define a methodology to produce and store small-scale microalgae paste to be used in a mollusk hatchery. Microalgae were cultured in 500 L fiberglass tanks, under temperature of 20 ¡À 2 C, Guillard f/2 culture medium, and continuous light intensity of 203¨C226 ¦Ìmol photons/m2/sec. Cultures were centrifuged at 2000 g at the exponential growth phase. Microalgae cell quality after centrifugation and during storage was determined by analyses with Evan¡¯s blue stain and by counting the number of total marine bacteria. Treatments with and without additive were applied to the microalgae paste produced, which was distributed into 100 mL plastic containers, capped, and stored under refrigeration at 4 ¡À 1 C. Results indicated that in the Chaetoceros muelleri paste, centrifugation did not damage the cells and the number of total marine bacteria reduced significantly from 2.9 ¡Á 106 to 8.3 ¡Á 105 colony-forming units per milliliter. Chaetoceros muelleri and Chaetoceros calcitrans pastes stored with addition of 0.1% ascorbic acid had a shelf life shorter than 2 wk. For the treatment without additive, results with Evan¡¯s blue stain showed that cells (99%) remained viable until the sixth week of storage for C. muelleri and seventh week of storage for Skeletonema sp. and C. calcitrans. The number of bacteria did not increase during storage for C. calcitrans and Skeletonema (P > 0.05). For C. muelleri, an increase in bacteria (P < 0.05) was observed afte... Mostrar Tudo |
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Palavras-Chave: |
C. muelleri; Chaetoceros calcitrans; Microalgae; Skeletonema sp. |
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Categoria do assunto: |
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Marc: |
LEADER 02301naa a2200181 a 4500
001 1077290
005 2011-06-07
008 2009 bl uuuu u00u1 u #d
100 1 $aEpagri
245 $aEvaluation of the Microalgae Paste Viability Produced in a Mollusk hatchery in Southern Brazil.
260 $c2009
500 $aISSN, 1749-7345
520 $aThe present study was conducted to define a methodology to produce and store small-scale microalgae paste to be used in a mollusk hatchery. Microalgae were cultured in 500 L fiberglass tanks, under temperature of 20 ¡À 2 C, Guillard f/2 culture medium, and continuous light intensity of 203¨C226 ¦Ìmol photons/m2/sec. Cultures were centrifuged at 2000 g at the exponential growth phase. Microalgae cell quality after centrifugation and during storage was determined by analyses with Evan¡¯s blue stain and by counting the number of total marine bacteria. Treatments with and without additive were applied to the microalgae paste produced, which was distributed into 100 mL plastic containers, capped, and stored under refrigeration at 4 ¡À 1 C. Results indicated that in the Chaetoceros muelleri paste, centrifugation did not damage the cells and the number of total marine bacteria reduced significantly from 2.9 ¡Á 106 to 8.3 ¡Á 105 colony-forming units per milliliter. Chaetoceros muelleri and Chaetoceros calcitrans pastes stored with addition of 0.1% ascorbic acid had a shelf life shorter than 2 wk. For the treatment without additive, results with Evan¡¯s blue stain showed that cells (99%) remained viable until the sixth week of storage for C. muelleri and seventh week of storage for Skeletonema sp. and C. calcitrans. The number of bacteria did not increase during storage for C. calcitrans and Skeletonema (P > 0.05). For C. muelleri, an increase in bacteria (P < 0.05) was observed after the sixth week of storage. This study demonstrated the feasibility to produce and store microalgae paste for a period of 2¨C8 wk, which allows it to be used as food source and also optimizes the use of microalgae cultured in laboratory.
653 $aC. muelleri
653 $aChaetoceros calcitrans
653 $aMicroalgae
653 $aSkeletonema sp
773 $tJournal of the World Aquaculture Society, USA$gv. 40, n. 1, p. 87-94, 2009.
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