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Registro Completo |
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Biblioteca(s): |
Epagri-Sede. |
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Data corrente: |
13/05/2009 |
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Data da última atualização: |
26/05/2011 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SILVEIRA, E. L. da; PEREIRA, R. M.; SCAQUITTO, D. C.; PEDRINHO, E. A. N.; VAL-MORAES, S. P.; WICKERT, E.; CARARETO-ALVES, L. M.; LEMOS, E. G. de M. |
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Afiliação: |
Epagri |
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Título: |
Bacterial diversity of soil under eucalyptus assessed by 16S rDNA sequencing analysis. |
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Ano de publicação: |
2006 |
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Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, v.41, n.10, p.1507-1516, out. 2006. |
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Idioma: |
Inglês |
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Conteúdo: |
Studies on the impact of Eucalyptus spp. on Brazilian soils have focused on soil chemical properties and isolating interesting microbial organisms. Few studies have focused on microbial diversity and ecology in Brazil due to limited coverage of traditional cultivation and isolation methods. Molecular microbial ecology methods based on PCR amplified 16S rDNA have enriched the knowledge of soils microbial biodiversity. The objective of this work was to compare and estimate the bacterial diversity of sympatric communities within soils from two areas, a native forest (NFA) and an eucalyptus arboretum (EAA). PCR primers, whose target soil metagenomic 16S rDNA were used to amplify soil DNA, were cloned using pGEM-T and sequenced to determine bacterial diversity. From the NFA soil 134 clones were analyzed, while 116 clones were analyzed from the EAA soil samples. The sequences were compared with those online at the GenBank. Phylogenetic analyses revealed differences between the soil types and high diversity in both communities. Soil from the Eucalyptus spp. arboretum was found to have a greater bacterial diversity than the soil investigated from the native forest area. |
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Palavras-Chave: |
Comunidade microbiana; Diversidade genética; Eucalipto; Genetic diversity; Metagenomic; Metagenômica; Microbial communities. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02042naa a2200289 a 4500
001 1063360
005 2011-05-26
008 2006 bl uuuu u00u1 u #d
100 1 $aSILVEIRA, E. L. da
245 $aBacterial diversity of soil under eucalyptus assessed by 16S rDNA sequencing analysis.
260 $c2006
520 $aStudies on the impact of Eucalyptus spp. on Brazilian soils have focused on soil chemical properties and isolating interesting microbial organisms. Few studies have focused on microbial diversity and ecology in Brazil due to limited coverage of traditional cultivation and isolation methods. Molecular microbial ecology methods based on PCR amplified 16S rDNA have enriched the knowledge of soils microbial biodiversity. The objective of this work was to compare and estimate the bacterial diversity of sympatric communities within soils from two areas, a native forest (NFA) and an eucalyptus arboretum (EAA). PCR primers, whose target soil metagenomic 16S rDNA were used to amplify soil DNA, were cloned using pGEM-T and sequenced to determine bacterial diversity. From the NFA soil 134 clones were analyzed, while 116 clones were analyzed from the EAA soil samples. The sequences were compared with those online at the GenBank. Phylogenetic analyses revealed differences between the soil types and high diversity in both communities. Soil from the Eucalyptus spp. arboretum was found to have a greater bacterial diversity than the soil investigated from the native forest area.
653 $aComunidade microbiana
653 $aDiversidade genética
653 $aEucalipto
653 $aGenetic diversity
653 $aMetagenomic
653 $aMetagenômica
653 $aMicrobial communities
700 1 $aPEREIRA, R. M.
700 1 $aSCAQUITTO, D. C.
700 1 $aPEDRINHO, E. A. N.
700 1 $aVAL-MORAES, S. P.
700 1 $aWICKERT, E.
700 1 $aCARARETO-ALVES, L. M.
700 1 $aLEMOS, E. G. de M.
773 $tPesquisa Agropecuária Brasileira, Brasília$gv.41, n.10, p.1507-1516, out. 2006.
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Epagri-Sede (Epagri-Sede) |
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| Registros recuperados : 4 | |
| 2. |  | PEDRINHO, E. N.; LEMOS, E. G. M.; PEREIRA, R. M.; SCAQUITTO, D. C.; SILVEIRA, E. L.; ALVES, L. M. C.; WICKERT, E.; VALARINI, M. J. Avaliação do impacto do lodo de esgoto na microbiota do solo utilizando o gene 16S rRNA. Arquivos do Instituto Biológico, São Paulo, v. 76, n. 3, p. 447-452, 2009.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: -- - -- |
| Biblioteca(s): Epagri-Sede. |
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| 3. | | PEREIRA, R. M.; SILVEIRA, E. L.; PEDRINHO, E. A. N.; VAL-MORAES, S. P.; WICKERT, E.; ALVES, L. M. C.; LEMOS, E. G. M. Molecular characterization of bacterial populations of diferent soils. Brazilian Journal of Microbiology, São Paulo, n. 37, p. 439-447, 2006.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: -- - -- |
| Biblioteca(s): Epagri-Sede. |
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| 4. | | SILVEIRA, E. L. da; PEREIRA, R. M.; SCAQUITTO, D. C.; PEDRINHO, E. A. N.; VAL-MORAES, S. P.; WICKERT, E.; CARARETO-ALVES, L. M.; LEMOS, E. G. de M. Bacterial diversity of soil under eucalyptus assessed by 16S rDNA sequencing analysis. Pesquisa Agropecuária Brasileira, Brasília, v.41, n.10, p.1507-1516, out. 2006.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: -- - -- |
| Biblioteca(s): Epagri-Sede. |
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| Registros recuperados : 4 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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